MAO Hong-mei, PING Yu-hui, ZONG Xing-xing, et al. Simaltanous Determination of Hederagenina,Oleanolic Acid and Ursolic Acid in Herba Patriniae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 89-92.
MAO Hong-mei, PING Yu-hui, ZONG Xing-xing, et al. Simaltanous Determination of Hederagenina,Oleanolic Acid and Ursolic Acid in Herba Patriniae by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 89-92. DOI: 10.13422/j.cnki.syfjx.2012.15.037.
Simaltanous Determination of Hederagenina,Oleanolic Acid and Ursolic Acid in Herba Patriniae by HPLC
Objective:To establish a method for the quantitative determination of hederagenin
oleanolic acid and ursolic acid in Herba Patriniae Simudtanoukly by HPLC.Method:The analysis was performed on Ultimate C18(4.6 mm×250 mm
5 μm) column with mobile phase consisted of methanol-0.2% ammonium acetat water by Gradient elution.The detected wavelength was set at 210 nm.The flow rate was 1.0 mL·min-1 and the column temperature was at 25 ℃.Result: The linearity was good in the ranges of 0.026-0.26
0.082-0.82 and 0.079-0.79 g·L-1 respectively for ederagenin
oaleanolic acid and ursolic acid(r>0.999 8).The average recoveries of hederagenin
oleanolic acid and ursolic acid were 100.62%(RSD 2.01%
n=6)
96.71%(RSD 1.89%
n=6) and 97.85%(RSD 2.17%
n=6).Conclusion: This method was highly sensitive
rapid
simple
specific
accurate and well represented
which can be used for the quality control of Herba Patriniae.