ZHANG Qing-he, LIU Hai-yu, LIN Mei-yu, et al. GC-MS Analysis of Volatile Oil Constituents of Yinhua Miyanling Tablet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 99-101.
ZHANG Qing-he, LIU Hai-yu, LIN Mei-yu, et al. GC-MS Analysis of Volatile Oil Constituents of Yinhua Miyanling Tablet[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 99-101. DOI: 10.13422/j.cnki.syfjx.2012.15.040.
GC-MS Analysis of Volatile Oil Constituents of Yinhua Miyanling Tablet
面积归一化法测定其相对含量。色谱柱为Agilent19091M-436弹性石英毛细管色谱柱(0.25 mm×30 m
0.25μm);程序升温
起始温度为60℃
以10℃.min-1升温至280℃
保持12 min;进样口温度为280℃。质谱条件:电子轰击(EI)离子源
电离电压70 eV
离子源温度230℃
扫描范围m/z 20~500。结果:共分离出30个化学成分
鉴定出其中的21种成分
相对含量超过5%的成分有棕榈酸(34.15%)、棕榈酸乙酯(21.01%)、1
5
9-三甲基-12-异丙烯基-1
5
9-十四烷环三烯(10.82%)、(Z
Z
Z)-9
12
15-亚油酸乙酯(7.71%)。结论:银花泌炎灵片中挥发油成分主要包含棕榈酸
棕榈酸乙酯
亚油酸乙酯类成分等。
Abstract
Objective: GC-MS was used to analyze the volatile oil components in Yinhua Miyanling tablet to enrich the study of chemical composition and provide a theoretical basis for quality control.Method: The volatile oil were extracted with steam distillation method according to ChP(2010).The chemical compositions were separated by GC capillary column chromatography
and the relative content of constituents were determined by area normalization method.The GC-MS analysis was performed on an Agilent 19091M-436 capillary column(0.25 mm×30 m
0.25 μm).The inlet temperature was maintained at 280 ℃.The column oven was begun at 60 ℃
then programmed from 60 to 280 ℃ at 10 ℃·min-1
held for 12 min.Mass spectrometer conditions were: ionization mode
EI electron;energy 70 eV;ion source temperature 230 ℃.The mass selective detector was operated in the TIC mode
mass scan range
20-500 amu.Result: Thiry compounds were detected and 21 compounds of them were identified.And the compounds that the relative content was more than 5% were palmitic acid(34.15%)
ethyl palmitate(21.01%) and 1
5
9-trimethyl-12-(1-methylethenyl)-1
5
9-cyclotetradecatriene(10.81%)
(Z
Z
Z)-9
12
15-ethyl linoleate(7.71%).Conclusion: The main components in the oil were palmitic acid