Determination of Amino Acids with OPA-FMOC by On-line Derivatization

SU Jian-kun; WANG Xue; LU Jian-qiu; QIAO Yan-jiang; GAO Xiao-yan

doi:10.13422/j.cnki.syfjx.2012.15.048

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Determination of Amino Acids with OPA-FMOC by On-line Derivatization

更新时间：2024-09-23

- Determination of Amino Acids with OPA-FMOC by On-line Derivatization
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 135-138(2012)
- 作者机构：
  
  北京中医药大学科研实验中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.048
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]苏建坤,王雪,卢建秋,乔延江,高晓燕.OPA-FMOC在线衍生化法测定氨基酸的含量[J].中国实验方剂学杂志,2012,18(15):135-138.

SU Jian-kun, WANG Xue, LU Jian-qiu, et al. Determination of Amino Acids with OPA-FMOC by On-line Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 135-138.
[1]苏建坤,王雪,卢建秋,乔延江,高晓燕.OPA-FMOC在线衍生化法测定氨基酸的含量[J].中国实验方剂学杂志,2012,18(15):135-138. DOI： 10.13422/j.cnki.syfjx.2012.15.048.

SU Jian-kun, WANG Xue, LU Jian-qiu, et al. Determination of Amino Acids with OPA-FMOC by On-line Derivatization[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 135-138. DOI： 10.13422/j.cnki.syfjx.2012.15.048.

摘要

目的:建立了测定氨基酸的在线衍生化方法。方法:以邻苯二甲醛（OPA）和9-芴甲基氯甲酸酯为衍生化试剂

对其氨基酸进行在线衍生化色谱分析。色谱柱:Zorbax Eclipse-AAA氨基酸分析柱（4.6 mm×150 mm

5μm）

流动相A为40mmol·L-1Na2HPO4（用8 mol·L-1氢氧化钠溶液调pH 7.8）

流动相B为乙腈-甲醇-水（45∶45∶10）梯度洗脱

流速2 mL·min-1

检测波长338

262 nm

柱温40℃。结果:氨基酸浓度在一定范围内

此法可以测定27种氨基酸的含量

但不适合清开灵注射液中氨基酸的含量测定。结论:该方法不能对清开灵注射液中氨基酸进行准确定量。

Abstract

Objective: To develop a method for determination of amino acids by on-line derivatization.Method: The amino acids were analyzed by HPLC with orthophthalaldehyde（OPA） and 9-formic acid methyl ester of fluorene chlorine（FMOC） on line derivatization.The sample was analyzed on a Zorbax Eclipse-AAA column（4.6 mm×150 mm

5 μm） with gradient elution of 40 mmol·L-1 Na2HPO4（make pH be 7.8 with 8 mol·L-1 NaOH）（A） and acetonitrile-methanol-water（45∶ 45∶ 10）（B） at the flow rate of 2.0 mL·min-1

detected at 338 nm and 262 nm.Result: This method could be used to analyze 27 amino acids in the range of certain concentration

but it wasn’t fit for the determination of amino acids in Qingkailing injection.Conclusion: This method couldn’t determine amino acids in Qingkailing injection accurately.

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Related Author

SU Jian-kun

WANG Xue

LU Jian-qiu

QIAO Yan-jiang

GAO Xiao-yan

SHAO Juan

XU Mengdan

ZHANG Xuejing

Related Institution

School of Pharmaceutical Sciences， Changchun University of Chinese Medicine

Jilin Ginseng Academy，Changchun University of Chinese Medicine

State Key Laboratory Breeding Base of Dao-di Herbs，National Resource Center for Chinese Materia Medica，China Academy of Chinese Medical Sciences

School of Pharmacy， Zhejiang Chinese Medical University

Chengdu Baoxiong Traditional Chinese Medicine Co. Ltd.，Pengzhou

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