Lipidomic Study of Protective Effect of Chaihuang Yishen Graunules on Rats with Diabetic Nephropathy

ZHAO Tie1; ZHANG Hao-jun2; YIN Ting-ting3; LU Xiao-guang2; ZHAO Ting-ting2; LI Ping2

doi:10.13422/j.cnki.syfjx.2012.15.052

您当前的位置：

首页 >

文章列表页 >

Lipidomic Study of Protective Effect of Chaihuang Yishen Graunules on Rats with Diabetic Nephropathy

更新时间：2024-09-23

- Lipidomic Study of Protective Effect of Chaihuang Yishen Graunules on Rats with Diabetic Nephropathy
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 155-162(2012)
- 作者机构：
  
  1. 中日友好医院药学部
  2. 中日友好医院临床医学研究所药物药理室
  3. 沈阳药科大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.052
  CLC： R285.5
- Published：2012
- 稿件说明：
移动端阅览
[1]赵铁,张浩军,殷婷婷,路晓光,赵婷婷,李平.柴黄益肾颗粒对糖尿病肾病大鼠保护作用的脂质组学研究[J].中国实验方剂学杂志,2012,18(15):155-162.

ZHAO Tie1, ZHANG Hao-jun2, YIN Ting-ting3, et al. Lipidomic Study of Protective Effect of Chaihuang Yishen Graunules on Rats with Diabetic Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 155-162.
[1]赵铁,张浩军,殷婷婷,路晓光,赵婷婷,李平.柴黄益肾颗粒对糖尿病肾病大鼠保护作用的脂质组学研究[J].中国实验方剂学杂志,2012,18(15):155-162. DOI： 10.13422/j.cnki.syfjx.2012.15.052.

ZHAO Tie1, ZHANG Hao-jun2, YIN Ting-ting3, et al. Lipidomic Study of Protective Effect of Chaihuang Yishen Graunules on Rats with Diabetic Nephropathy[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 155-162. DOI： 10.13422/j.cnki.syfjx.2012.15.052.

摘要

目的:研究柴黄益肾颗粒干预对糖尿病肾病（DN）大鼠肾脏脂质代谢异常的调节作用。方法:采用单侧肾切除加i.p.链脲佐菌素诱导的方法复制DN大鼠模型

将38只Wistar大鼠分成4组

包括空白组、模型组、模型+柴黄益肾颗粒组、空白+柴黄益肾颗粒组。模型+柴黄益肾颗粒组ig给予0.56 g·kg-1柴黄益肾颗粒

于造模成功后第20周

采集肾皮质样品进行基于超高压液相-四级杆飞行时间质谱联用仪（UPLC-QTOF MS）的脂质组学研究

考察干预前后脂质代谢物组的变化。结果:造模成功第20周时

大鼠肾皮质中多种甘油磷脂酰胆碱、甘油磷脂酰乙醇胺、甘油磷脂酰甘油醇、甘油磷脂酰肌醇、鞘磷脂酰胆碱等都显著下调

而胆固醇酯和甘油三酯则显著上升。柴黄益肾颗粒干预对部分异常脂质代谢表现出明显的改善作用

包括4种鞘磷脂酰胆碱和2种甘油磷脂酰胆碱

即SM（d18∶1/16∶1）

SM（C33∶1）

SM（C42∶2）

SM（C42∶3）

PC（36∶4）和PC（42∶10）

其相对变化倍数分别为1.38

1.50

1.29

1.23

1.15和1.30。结论:柴黄益肾颗粒干预可以延缓大鼠DN的进展

该机制可能与其对脂质尤其是鞘脂代谢异常的调节有关。

Abstract

Objective∶ To study the protective effect of Chaihuang Yishen granules on dysregulated lipids metabolism in kidney of rats with diabetic nephropathy（DN）.Method: Wistar rats were undergone uninephrectomy and a single intraperitoneal injection of streptozocin（STZ） to induce diabetes

and administratered with 0.56 g·kg-1 Chaihuang Yishen granules.At the end of 20 weeks

renal cortex of rats was removed and UPLC-QTOF MS was used to lipidomic study and to investigate the alterations of abnormal lipidome after administration of Chaihuang Yishen granules.Result: At the end of 20 weeks

multiple species of significantly decreased glycerophosphocholines

glycerophosphoethanolamines

glycerophosphoglycerols

glycerophosphoinositols and sphingomyelins

and clearly elevated cholesterol esters and triglycerides were identified in renal cortex of rats with DN.Among them

several lipid metabolites

such as 2 glycerophosphocholines and 4 sphingomyelins-SM（d18∶ 1/16∶ 1）

SM（C33∶ 1）

SM（C42∶ 2）

SM（C42∶ 3）

PC（36∶ 4） and PC（42∶ 10）

can be normalized towards their baseline by the intervention of Chaihuang Yishen granules with individually relative fold change: 1.38

1.50

1.29

1.23

1.15 and 1.30.Conlusion: Chaihuang Yishen gruanules can retard the development of DN in rats

which might be associated with its regulation in abnormally expressed lipids

especially sphingomyelins.

关键词

Keywords

references

Views

687

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Mechanism of HIF-1α in Diabetic Nephropathy and Improvement Effect of Traditional Chinese Medicine： A Review

Randomized Controlled Study of Baoshen Prescription in Treating Stage Ⅳ Diabetic Nephropathy in Patients with Syndromes of Qi-Yin Deficiency and Kidney Collateral Stasis and Obstruction

Exploring Therapeutic Effect of Yuejuwan on Depressed Mice Based on Lipidomics

Mechanism of Action of Coptidis Rhizoma and Ophiopogonis Radix in Delaying Diabetic Nephropathy Based on EGFR/PI3K/Akt Signaling Pathway

Related Author

ZHAO Tie

ZHANG Hao-jun

YIN Ting-ting

LU Xiao-guang

ZHAO Ting-ting

LI Ping

XIE Jiarun

LIN Haoyu

Related Institution

School of Traditional Chinese Medicine，Southern Medical University

Zhujiang Hospital of Southern Medical University

Beijing Hospital of Traditional Chinese Medicine（TCM） Affiliated to Capital Medical University

ZHANG Binghou Studio of National Famous TCM Inheritance

Key Laboratory of Depression Animal Model Based on Traditional Chinese Medicine（TCM） Syndrome of Jiangxi Administration of TCM，Key Laboratory of Evaluation of TCM Efficacy （Prevention and Treatment of Brain Diseases with Mental Disorders） of Jiangxi Administration of TCM，Jiangxi University of Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰