LI Zhi-yi1, CUI Li-fang2, ZHANG Ling-li3, et al. Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 192-195.
LI Zhi-yi1, CUI Li-fang2, ZHANG Ling-li3, et al. Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 192-195. DOI: 10.13422/j.cnki.syfjx.2012.15.060.
Effect of Biejiajian Wan on Hepatocyte Growth Factor Expression in Rats with Immune Hepatic Fibrosis
Objective: To observe the effects of Biejiajian Wan(BJJW) on hepatocyte growth factor(HGF) expression in the rats with immune hepatic fibrosis
and analyze therapeutic functions of anti-hepatic fibrosis of BJJW and explore the mechanism.Method: Ninety male Wistar rats were randomly divided into six groups: normal control group
model control group
colchicine prevention group
colchicine treatment group
BJJW prevention group
BJJW treatment group.The rat liver fibrosis model was produced by porcine serum.After 10 weeks eight rats from each group were selected randomly.The hepatic tissues of the rats were taken and stained by immunohistochemistry
HGF of them was detected by image analysis.Hyaluronic acid(HA)
laminin(LN)
collagen type Ⅲ(PCⅢ)
type Ⅳ collagen(Ⅳ-C)in serum.Result: Compared with model group
the level of HA
LN
PCⅢ
Ⅳ-C was obviously decreased in preventive groups and therapeutic groups
but BJJW preventive groups and therapeutic groups decreased most significantly.The expression of HGF were obviously increased in preventive groups and therapeutic groups
but BJJW preventive and therapeutic groups increased most significantly.Conclusion: BJJW can inhibit the rat’s pathological changes of hepatic fibrosis
significantly increase the expression of HGF
which indicates the obvious function of anti-hepatic fibrosis.