Effect of Icariin on Rats Femur Tissue in vitro

WANG Jia-qi1; LI Xue-yan1; WU He-long1; CHEN Ke-ming2

doi:10.13422/j.cnki.syfjx.2012.15.061

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Effect of Icariin on Rats Femur Tissue in vitro

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- Effect of Icariin on Rats Femur Tissue in vitro
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 15, Pages: 196-200(2012)
- 作者机构：
  
  1. 兰州理工大学生命科学与工程学院
  2. 兰州军区兰州总医院骨科研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.15.061
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]王嘉琪,李雪雁,吴河龙,陈克明.淫羊藿苷对体外培养大鼠股骨组织的影响[J].中国实验方剂学杂志,2012,18(15):196-200.

WANG Jia-qi1, LI Xue-yan1, WU He-long1, et al. Effect of Icariin on Rats Femur Tissue in vitro[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 196-200.
[1]王嘉琪,李雪雁,吴河龙,陈克明.淫羊藿苷对体外培养大鼠股骨组织的影响[J].中国实验方剂学杂志,2012,18(15):196-200. DOI： 10.13422/j.cnki.syfjx.2012.15.061.

WANG Jia-qi1, LI Xue-yan1, WU He-long1, et al. Effect of Icariin on Rats Femur Tissue in vitro[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(15): 196-200. DOI： 10.13422/j.cnki.syfjx.2012.15.061.

摘要

目的:研究淫羊藿苷对体外培养大鼠股骨组织的影响。方法:无菌条件下体外分离培养大鼠股骨组织

采用培养液中终浓度为1×10-5mol·L-1的淫羊藿苷进行药物干预

检测碱性磷酸酶活性（alkaline phosphatase

ALP）、钙盐沉积量以及Real-Time RT-PCR检测骨保护素（osteoprotegerin

OPG）

Ⅰ-型胶原（collagen-1）

Runt-related transcription factor 2（Runx-2）mRNA的表达情况。结果:1×10-5mol·L-1淫羊藿苷处理组ALP活性从第6～12天呈升高趋势

第12～18天降低;6～15 d各组钙盐沉积量呈升高趋势

第18天降低;淫羊蒮苷组处理股骨组织2～4 d时促进Runx-2 mRNA的表达水平

处理8 d时抑制Runx-2 mRNA的表达;淫羊蒮苷组处理股骨组织2～8 d时促进Collage-1 mRNA的表达水平;淫羊蒮苷组处理股骨组织2～4 d时能促进OPG mRNA的表达水平

处理6

8 d时与对照组比较无统计学意义。结论:淫羊藿苷可显著提高体外培养大鼠股骨组织中骨代谢相关基因的表达水平、增加钙盐沉积量以及ALP活性。

Abstract

Objective: To investigate the effects of icariin on the femur tissue in vitro.Method: The rat femur tissue was isolated from SD rats with enzyme digestion.Icariin was supplemented into the culture at 1×10-5 mol·L-1

alkaline phosphatase（ALP） activity and calcium content were compared between the icariin group and the control group.Total RNA was isolated and the gene expression of osteoprotegerin（OPG）

collagen-1

Rurt-related transcription factor 2（Runx-2） was detected by Real-time RT-PCR.Result: 1×10-5 mol·L-1 icariin significantly promoted femur tissue metabolism related gene expression level

increased ALP activity and calcium content.Conclusion: Icariin can enhance the rat femur tissue in bone metabolism related gene expression

increase calcium content and ALP activity.

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Related Author

WANG Jia-qi

LI Xue-yan

WU He-long

CHEN Ke-ming

DU Jianqiang

QIN Wenxiu

YIN Xuesong

ZHAO Dan

Related Institution

Binhai New Area Hospital of TCM（Fourth Teaching Hospital of Tianjin University of TCM）

Gansu University of Chinese Medicine

The Second Affiliated Hospital of Tianjin University of TCM

Tianjin University of Traditional Chinese Medicine（TCM）

Graduate School，Beijing University of Chinese Medicine

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