Study of Zengye Bujin Decoction on Mice Model with Sjgren’s Syndrome

HE Hui-zhen; JIN Gui-lan; SUN Li-xia; TONG Yong-zhong

doi:10.13422/j.cnki.syfjx.2012.16.041

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Study of Zengye Bujin Decoction on Mice Model with Sjgren’s Syndrome

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- Study of Zengye Bujin Decoction on Mice Model with Sjgren’s Syndrome
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 208-212(2012)
- 作者机构：
  
  南京中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.041
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]何慧珍,金桂兰,孙丽霞,童永忠.增液布津汤对干燥综合征模型鼠治疗作用的研究[J].中国实验方剂学杂志,2012,18(16):208-212.

HE Hui-zhen, JIN Gui-lan, SUN Li-xia, et al. Study of Zengye Bujin Decoction on Mice Model with Sjgren’s Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 208-212.
[1]何慧珍,金桂兰,孙丽霞,童永忠.增液布津汤对干燥综合征模型鼠治疗作用的研究[J].中国实验方剂学杂志,2012,18(16):208-212. DOI： 10.13422/j.cnki.syfjx.2012.16.041.

HE Hui-zhen, JIN Gui-lan, SUN Li-xia, et al. Study of Zengye Bujin Decoction on Mice Model with Sjgren’s Syndrome[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 208-212. DOI： 10.13422/j.cnki.syfjx.2012.16.041.

摘要

目的:观察增液布津汤对干燥综合征（Sjgren’s syndrome

SS）模型鼠的治疗作用。方法:BALB/c小鼠60只

随机分为正常对照组、模型组、增液布津汤低、中、高剂量组和阳性药环戊硫酮组。除正常组外

其余5组均利用免疫诱导法（注射白百破疫苗+完全弗氏佐剂+大鼠颌下腺抗原）建立干燥综合征小鼠模型

于造模后当天正常对照组及模型组用生理盐水20 mL.kg-1ig

中药低、中、高剂量组用增液布津汤（相当于生药6.8

13.6

27.3 g.kg-1）ig

环戊硫酮组用环戊硫酮（1.25 g.kg-1）ig。首次免疫后第3

7天

用乳化抗原对模型组和用药组加强免疫

剂量同首次

多点背部sc。以后每隔14 d用乳化抗原加强免疫1次

剂量同首次

共加强免疫2次

多点背部sc

正常对照组不作任何处理。观察增液布津汤对模型小鼠体质量、颌下腺与胸腺、脾指数及唾液分泌量、颌下腺组织形态学变化的影响。结果:免疫小鼠颌下腺出现类似SS的病理改变

存在大量淋巴细胞浸润

提示免疫造模成功。模型组小鼠的体质量、唾液流量分别为（18.17±1.33）g

（7.51±1.25）mg

颌下腺指数、胸腺指数、脾指数分别为（3.02±0.98）

（1.76±0.62）

（6.93±2.03）mg.g-1

与正常对照组相比有显著性差异（P<0.01）。增液布津汤高剂量组小鼠体质量、唾液流量分别为（23.79±1.48）g

（11.05±1.93）mg

颌下腺指数、胸腺指数、脾指数分别为（4.24±0.84）

（0.77±0.70）

（4.58±1.39）mg.g-1

与模型组相比有显著性差异（P<0.01）

余治疗组增加程度较增液布津汤高剂量组相比增加不明显。结论:增液布津汤可能通过抑制SS模型小鼠颌下腺萎缩

淋巴细胞浸润的病理进程

保护其唾液分泌功能

从而发挥对SS模型小鼠的治疗作用。

Abstract

Objective:To observe the therapeutic effect of Zenye Bujin decoction on the mice model of Sjgren’s syndrome.Method: BALB/c rats were divided into normal group

model group

Zengye Bujin decoction low dose group

Zengye Bujin decoction middle dose group

Zengye Bujin decoction high dose group

anethol trithione group.In addition to normal group

other five groups were induced by immune inducement method（injection of pertussis vaccine

complete Freund’s adjuvant and mice glandula angularis antigen）.Then the normal group and model groupwere given normal saline gavage

Zengye Bujin decoction low dose（6.8 g ·kg-1）

middle dose（12.6 g · kg-1）

high dose（27.3 g · kg-1）were given treatment groups

and anethol trithione（1.25 g ·kg-1） was given anethol trithione group.After first immune

at 3th and 7th day emulsifier antigens was use to reinforce immune

the same dose as the first

sc on back.The changes of wight food and water intakes were observe every two days

saliva flow rate.Thymus gland index

spleen gland index

submaxillary gland index and pathological changes of submaxillary were also observed.Result: After the experiment

pathological changes were similar to the salivary gland in human SS were found.Compared with normal group

the weight and saliva flow rate of modle group decreased（P<0.01）.Compared with model group

the high dosage group of Zenye Bujin decoction could increase body weight

submaxillary gland index and saliva flow rate

reduce thymus gland index

and relieve the pathological changes（P<0.01）.Conclusion: Zenye Bujin decoction may play a therapeutic role by inhibiting the submandibular gland atrophy

lymphocyte infiltration of the pathological process

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