Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis

HAO Rui-chun1; MEN Jiu-zhang1; LI Xia1; LI Xiao-bo1; LV Jian-jun2; LIANG Rui1

doi:10.13422/j.cnki.syfjx.2012.16.044

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Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis

更新时间：2024-09-23

- Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 219-221(2012)
- 作者机构：
  
  1. 山西中医学院
  2. 山西生物应用职业技术学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.044
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]郝瑞春,门九章,李霞,李孝波,吕建军,梁锐.雄芍汤对免疫性肝纤维化大鼠MMP-9和TIMP-1的影响[J].中国实验方剂学杂志,2012,18(16):219-221.

HAO Rui-chun1, MEN Jiu-zhang1, LI Xia1, et al. Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 219-221.
[1]郝瑞春,门九章,李霞,李孝波,吕建军,梁锐.雄芍汤对免疫性肝纤维化大鼠MMP-9和TIMP-1的影响[J].中国实验方剂学杂志,2012,18(16):219-221. DOI： 10.13422/j.cnki.syfjx.2012.16.044.

HAO Rui-chun1, MEN Jiu-zhang1, LI Xia1, et al. Effects of Xiongshao Decoction on MMP-9 and TIMP-1 in Rats with Immune Liver Fibrosis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 219-221. DOI： 10.13422/j.cnki.syfjx.2012.16.044.

摘要

目的:研究雄芍汤对肝纤维化大鼠基质金属蛋白酶-9（MMP-9）和金属蛋白酶组织抑制因子-1（TIMP-1）的影响。方法:大鼠分为正常组、模型组、扶正化瘀胶囊对照组（0.52520g.kg-1）、雄芍汤预防组（7.920g.kg-1）和高、低剂量组（15.8

7.9g.kg-1）。采用猪血清腹腔注射法复制大鼠肝纤维化模型。酶联免疫吸附（ELISA）法检测大鼠血清MMP-9含量

实时荧光定量PCR技术检测肝组织中TIMP-120mRNA的表达。结果:雄芍汤7.920g.kg-1剂量预防组MMP-9含量显著高于模型组（P<0.01）。雄芍汤15.820g.kg-1剂量组TIMP-120mRNA表达显著低于模型组（P<0.01）。结论:雄芍汤可以促进免疫性肝纤维化大鼠血清MMP-9生成

抑制大鼠肝组织TIMP-120mRNA表达。调节MMP与TIMP的协调平衡可能是雄芍汤抗肝纤维化的机制之一。

Abstract

Objective:Tostudy the effects of Xiongshao decoction（XSD）on matrix metalloproteinase 9（MMP-9） and tissue inhibitor of metalloproteinases 1（TIMP-1）in rats with liver fibrosis.Method: Eighty-four SD rats were randemly divided into normal group

model group

Fuzheng Huayu capsule group（0.525 g·kg-1）

XSD preservation group（7.9 g · kg-1）

high and low doses of XSD group（15.8

7.9 g · kg-1）.The hepatic fibrosis model was induced by injecting porcine serum to the abdominal cavity of rats.The levels of MMP-9 was detected by ELISA.The content of TIMP-1mRNA in hepatic tissue was detected by real-time fluorescence quantitative PCR.Result: The content of MMP-9 was obviously higher in XSD preservation group（7.9 g · kg-1） than in model group（P<0.01）.The content of TIMP-1mRNA was obviously lower in high-dose XSD group（15.8 g · kg-1） than in model group（P<0.01）.Conclusion: XSD can promote the production of MMP-9 and suppress the expression of TIMP-1 mRNA.Regulating the balance between MMPs and TIMPs is one of the anti-hepatic fibrosis mechanisms of XSD.

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