Objective:To observe the effect of Wuzi Yanzong Wan on the intercourse capacity of rats and the kidney-yang deficiency mice and investigate its pharmacological action of invigorating kidney and strengthening Yang.Method: ①Male Wistar rats were randomly divided into the normal control group

Nanbao group（0.35 g · kg-1）

Wuzi Yanzong Wan group（2.0

1.0

0.5 g · kg-1）.The rats were administrated daily by oral for 28 days.After the female rat was crated to the container with the male rat

the mating capability of male rat was evaluated by the catching latency

ejaculation latency

capture times and ejaculation frequency.Meanwhile

the content of testesterone（T）

and luteinizing hormone（LH） in rat serum were detected;②Male ICR mice were divided randomly into normal group

model group

Nanbao group（0.35 g · kg-1）

Wuzi Yanzong Wan group（2.4

1.2

0.6 g · kg-1）.Except for the normal group

the other groups were treated by hydrocortisone for a week.At 7 days post treatment with drug

the weight

body temperature

the numbers of autonomic activity and the survival time of swimming with low temperature were recorded.The spleen

thymus and genitals were weight to calculate the viscera index.Result: Wuzi Yanzong Wan can increase the catching numbers

ejaculation frequency and epididymis index significantly（P<0.05-P<0.01）.The content of T in blood has a rising trend while the LH was decreased significantly（P<0.01-P<0.001） compared to the control group.Wuzi Yanzong Wan can prolong its swimming endurance in low temperature（P<0.01-0.001） and elevate the body temperature and the numbers of autonomic activity.Besides

the spleen weight and he index of levator ani muscle were increased（P<0.05）.The spermary weight and the index of epididymis has a increasing trend.Conclustion: Wuzi Yanzong Wan have the function of building kidney and strengthening yang which can improve the male sexual function and have a significant effect on cold-resist and anti-fatigue.It was demonstrated that can improve the syndrome of kidney Yang deficiency.