Inhibition Macrophages Activation and Pro-inflammatory Cytokines of Xiaohuang Decoction in Dimethylnitrosamine-induced Liver Fibrosis in Rats

LIU Cheng1; CHEN Xiao-rong1; YANG Zong-guo1; LU Yun-fei1; LV Zhen1; XU Qing-nian1; PAN Qi1; TANG Bo-zong1; LV Ying1; SUN Yuan1; ZHANG Wen-si1; LU Ling-qing1

doi:10.13422/j.cnki.syfjx.2012.16.053

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Inhibition Macrophages Activation and Pro-inflammatory Cytokines of Xiaohuang Decoction in Dimethylnitrosamine-induced Liver Fibrosis in Rats

更新时间：2024-09-23

- Inhibition Macrophages Activation and Pro-inflammatory Cytokines of Xiaohuang Decoction in Dimethylnitrosamine-induced Liver Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 255-260(2012)
- 作者机构：
  
  1. (复旦大学附属)公共卫生临床中心中医科,上海市
  2. (复旦大学附属)公共卫生临床中心科研中心,上海市
  3. 国家中医药管理局中医药防治传染病重点研究室(临床基地)
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.053
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]刘成,陈晓蓉,杨宗国,陆云飞,吕震,徐庆年,潘奇,汤伯宗,吕莹,孙媛,张文思,陆玲庆.消黄方对二甲基亚硝胺致肝纤维化大鼠肝脏巨噬细胞及促炎症因子的干预作用[J].中国实验方剂学杂志,2012,18(16):255-260.

LIU Cheng1, CHEN Xiao-rong1, YANG Zong-guo1, et al. Inhibition Macrophages Activation and Pro-inflammatory Cytokines of Xiaohuang Decoction in Dimethylnitrosamine-induced Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 255-260.
[1]刘成,陈晓蓉,杨宗国,陆云飞,吕震,徐庆年,潘奇,汤伯宗,吕莹,孙媛,张文思,陆玲庆.消黄方对二甲基亚硝胺致肝纤维化大鼠肝脏巨噬细胞及促炎症因子的干预作用[J].中国实验方剂学杂志,2012,18(16):255-260. DOI： 10.13422/j.cnki.syfjx.2012.16.053.

LIU Cheng1, CHEN Xiao-rong1, YANG Zong-guo1, et al. Inhibition Macrophages Activation and Pro-inflammatory Cytokines of Xiaohuang Decoction in Dimethylnitrosamine-induced Liver Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 255-260. DOI： 10.13422/j.cnki.syfjx.2012.16.053.

摘要

目的:观察消黄方对二甲基亚硝胺（DMN）大鼠肝纤维化模型肝纤维化过程中肝脏巨噬细胞及促炎相关因子的干预作用。方法:每周320d连续腹腔注射DMN（1020mg.kg-1）

4周后制备大鼠肝纤维化模型。造模2周末将DMN大鼠随机分为模型组和消黄方组（每组10只）

造模第3周起消黄方组大鼠造模的同时每天给予消黄方1.73g.kg-1

灌胃至4周末

模型大鼠每天给予双蒸水。留取肝组织样本及血清

免疫印迹和免疫组化及实时定量PCR检测巨噬细胞标志物CD68及肿瘤坏死因子（TNF-α）

白介素1β（IL-1β）表达。结果:免疫组化结果显示

正常组CD68主要分布在肝窦

小叶间也有零星分布

DMN造模后CD68呈强阳性表达;实时定量PCR结果显示

与正常组相比

DMN造模4周CD68表达显著显著升高（P<0.01）;肝脏TNF-α和IL-1βmRNA与CD68表达一致。与模型相比

消黄方显著抑制CD68及TNF-α及IL-βmRNA表达（P<0.01或P<0.01）)。结论:消黄方对炎症反应的抑制作用可能其是发挥抗DMN肝纤维化效应机制之一。

Abstract

Objective:To investigate effects of Xiaohuang decoction on macrophages activation and pro-inflammatory cytokines in rats with dimethylnitrosamine（DMN）-induced liver fibrosis.Method: In DMN-treated rats

DMN（10 mg · kg-1） was administrated intraperitoneally for the first three days per week for 4 weeks.At the second weekend

DMN rats were randomly divided into DMN-water and DMN-Xiaohuang decoction（DMN-XHD）.At the first day of the third week

both groups continued to receive weekly DMN treatment for another 2 weeks in addition to daily administration of either water or XHD.CD68 was detected using western blotting

immunohistochemistry and real-time PCR.Result:The immunohistochemistry results showed that CD68 mainly located in the sinusoids and between interlobular in normal group.The CD68 mainly located in the fibrotic septa in DMN-treatment rats.The CD68 expression was increased 5-fold compared with normal rats showed by real-time PCR analysis.Compared with DMN-water rats

CD68 was reduced significantly in XHD rats.The content of Tumor necrosis factor（TNF-α） in the serum increased remarkably in DMN-water rats

and XHD could suppress TNF-α notably.Conclusion: Xiaohuang decoction inhibited liver macrophage activation and pro-inflammatory cytokines in DMN-induced liver fibrosis.

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