Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin

LI Chun-hong1; MEI Zhi-qiang2; TIAN Ji1; LIU Yan1

doi:10.13422/j.cnki.syfjx.2012.16.060

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Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin

更新时间：2024-09-23

- Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 27-29(2012)
- 作者机构：
  
  1. 泸州医学院药物与功能性食品研究中心
  2. 泸州医学院医学分子生物学教研室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.060
  CLC： R284
- Published：2012
- 稿件说明：
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[1]李春红,梅志强,田吉,刘艳.大孔树脂对黄独总皂苷的分离纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):27-29.

LI Chun-hong1, MEI Zhi-qiang2, TIAN Ji1, et al. Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 27-29.
[1]李春红,梅志强,田吉,刘艳.大孔树脂对黄独总皂苷的分离纯化工艺优选[J].中国实验方剂学杂志,2012,18(16):27-29. DOI： 10.13422/j.cnki.syfjx.2012.16.060.

LI Chun-hong1, MEI Zhi-qiang2, TIAN Ji1, et al. Optimization of Separation and Purification of Total Saponins from Dioscorea bulbifera by Macroporous Resin[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 27-29. DOI： 10.13422/j.cnki.syfjx.2012.16.060.

摘要

目的:优选黄独总皂苷的分离纯化工艺。方法:以黄独总皂苷含量为指标

采用紫外分光光度法测定其含量

动态吸附-解吸法筛选大孔树脂型号

并优选黄独总皂苷的分离纯化工艺。结果:D-101型大孔树脂富集纯化黄独总皂苷的最佳工艺为黄独提取液质量浓度0.2 g.mL-1

上样量6 BV

流速1.0 mL.min-1

加4 BV水洗除杂

50%乙醇4 BV解吸

收集洗脱液。此工艺条件下所得提取物黄独总皂苷质量分数61.85%。结论:该优选工艺简单可行

能满足大生产实际要求。

Abstract

Objective:To optimize separation and purification technology of total saponins from Dioscorea bulbifera.Method: With the content of total saponins from D.bulbifera as index

which was determined by ultraviolet spectrophotometry

optimum macroporous resin model was selected by dynamic adsorption-desorption method

then separation and purification technology of total saponins from D.bulbifera was optimized.Result: Optimum separation and purification technology conditions of total saponins by D-101 macroporous resin were as follows:the concentration of extraction liquid from D.bulbifera 0.2 g · mL-1

sample volume of 6 BV

flow rate of 1.0 mL · min-1

washed impurity with 4 BV water

and then desorpted with 4 BV 30% ethano1

collected eluate.Under these technology conditions

yield of total saponins from D.bulbifera reached as high as 61.85%.Conclusion: This optimized technology was simple and feasible

which could meet industrial requirements.

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Related Author

LI Chun-hong

MEI Zhi-qiang

TIAN Ji

LIU Yan

WANG Chang-ling

ZHU Xiang

FU Yan-ling

HU Kai-wen

Related Institution

College of Food and Biological Engineering，The Chongqing Engineering Laboratory for Green Cultivation and Deep Processing of the Three Gorges Reservoir Area's Medicinal Herbs， Chongqing Three Gorges University

School of Pharmacy，Tianjin University of Traditional Chinese Medicine

School of Pharmacy， Jiangxi University of Chinese Medicine

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Changchun University of Chinese Medicine

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