Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method

GUO Xiao-na; CHEN Wen; ZHANG Hua; REN Quan-xia; LIU Shan-shan

doi:10.13422/j.cnki.syfjx.2012.16.071

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Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method

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- Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 66-69(2012)
- 作者机构：
  
  新疆石河子大学药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.071
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]郭小娜,陈文,张华,任全霞,刘珊珊.GC内标法同时测定刺山柑果实中3种刺激性成分的含量[J].中国实验方剂学杂志,2012,18(16):66-69.

GUO Xiao-na, CHEN Wen, ZHANG Hua, et al. Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 66-69.
[1]郭小娜,陈文,张华,任全霞,刘珊珊.GC内标法同时测定刺山柑果实中3种刺激性成分的含量[J].中国实验方剂学杂志,2012,18(16):66-69. DOI： 10.13422/j.cnki.syfjx.2012.16.071.

GUO Xiao-na, CHEN Wen, ZHANG Hua, et al. Determination of Three Irritant Constituents of Cappers spinoza with Capillary Gas Chromatography by Using Internal Standard Method[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 66-69. DOI： 10.13422/j.cnki.syfjx.2012.16.071.

摘要

目的:建立毛细管气相色谱法同时测定刺山柑果实中3种刺激性成分（异硫氰酸异丙酯、异硫氰酸甲酯、异硫氰酸异丁酯）的方法。方法:采用挥发油提取器提取挥发油;以异硫氰酸丁酯为内标物

用TM-FFAP毛细管柱分离

火焰离子化检测器检测;以保留时间定性

内标法定量。结果:在所选的色谱条件下

上述成分在46 min内获得较好的分离;3种成分与内标物的峰面积比值与其浓度呈良好的线性关系（r>0.999 1）;样品加标平均回收率为85.9%～104%

RSD 2.44%～3.12%。结论:方法简单、准确

可同时测定刺山柑果实中3种刺激性成分的含量

为其质量控制提供依据。

Abstract

Objective:A capillary gas chromatographic method for the simultaneous determination of three irritant constituents（isopropyl isothiocyanate

methyl isothiocyanate

isobutyl isothiocyanate） in Cappers spinoza was developed.Method: The essential oil was extracted by steam distillation.Butyl isothiocyanate was selected as the internal standard substance.The contents of three irritant components was analyzed by gas chromatography using a TM-FFAP capillary column and a flame ionization detector.The retention time of the peak was applied for qualitative analysis

and the internal standard method was used for quantitative analysis.Result: Three components were well separated in 46 min.There were good linear relationships between peak area ratio on the mass concentration and correlation coefficents above 0.999 1.The average recoveries for the added standards and the RSD were 85.9%-104% and 2.44%-3.12%

respectively.Conclusion: This method is simple and accurate for the irritation determination of volatile substances of C.Spinoza.

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