Qualitative and Quantitative Determination of Clenbuterol Hydrochloride in Chinese Patent Medicines by UPLC-MS

FAN Lei-lei; LIU Nai-qiang; LI Zhen-guo

doi:10.13422/j.cnki.syfjx.2012.16.074

您当前的位置：

首页 >

文章列表页 >

Qualitative and Quantitative Determination of Clenbuterol Hydrochloride in Chinese Patent Medicines by UPLC-MS

更新时间：2024-09-23

- Qualitative and Quantitative Determination of Clenbuterol Hydrochloride in Chinese Patent Medicines by UPLC-MS
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 81-83(2012)
- 作者机构：
  
  河南省食品药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.074
  CLC： R286.0
- Published：2012
- 稿件说明：
移动端阅览
[1]樊磊磊,刘乃强,李振国.UPLC-MS定性定量检测中成药中非法添加的盐酸克仑特罗[J].中国实验方剂学杂志,2012,18(16):81-83.

FAN Lei-lei, LIU Nai-qiang, LI Zhen-guo. Qualitative and Quantitative Determination of Clenbuterol Hydrochloride in Chinese Patent Medicines by UPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 81-83.
[1]樊磊磊,刘乃强,李振国.UPLC-MS定性定量检测中成药中非法添加的盐酸克仑特罗[J].中国实验方剂学杂志,2012,18(16):81-83. DOI： 10.13422/j.cnki.syfjx.2012.16.074.

FAN Lei-lei, LIU Nai-qiang, LI Zhen-guo. Qualitative and Quantitative Determination of Clenbuterol Hydrochloride in Chinese Patent Medicines by UPLC-MS[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 81-83. DOI： 10.13422/j.cnki.syfjx.2012.16.074.

摘要

目的:建立UPLC-MS方法

快速定性定量检测中成药中非法添加的盐酸克仑特罗。方法:采用超高效液相色谱串联三重四极杆质谱仪。色谱条件:Agela Venusil MP-C18色谱柱（2.1 mm×50 mm

3μm）

柱温35℃

流动相甲醇-0.2%甲酸水溶液（50∶50）

流速0.3 mL.min-1。质谱条件:ESI源

多反应检测（MRM）。以保留时间及定性离子对之间的相对丰度定性

以定量离子对峰面积定量。结果:盐酸克仑特罗在1.092～27.300μg.L-1呈现良好的线性关系（r>0.999 8）;平均加样回收率97.26%;定量限为0.19 pg。结论:本方法定性可靠、定量准确

且快速简便

可用于中成药非法添加盐酸克仑特罗化学药品的检测。

Abstract

Objective:To establish an UPLC-MS method for determination of Clenbuterol hydrochloride in Chinese patent medicines.Method: Ultra performance liquid chromatography tandem three quadrupole mass spectrometer was adopted

LC condition:A agela Venusil MP-C18（2.1 mm×50 mm

3 μm） column was used with the isocrstic elution of methanol and 0.2% methanoic acid at a flow rate 0.3 mL · min-1.MS condition: adopted electrospray ionization（ESI）

and multiple reaction monitoring

The relative intensities of the detected qualitative ion pairs using for qualitative

integrating the signals of the detected quantitative ion pairs using for quantitative.Result: The clenbuterol hydrochloride had good linearity with correlation coefficient>0.999 8

ranging from 1.092-27.300 μg · L-1.The average recoveries were 97.26%.The limit of quantitation was 0.19 pg.Conclusion: This method is reliable qualitative and quantitative accuracy

simple and rapid

and can be used for proprietary Chinese medicines illegally added clenbuterol hydrochloride detection.

关键词

Keywords

references

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Qualitative and Quantitative Determination of Antidiabetic Drugs in Wentangan Capsule by UPLC-MS

Simultaneous Determination of 9 Illegally Added Anti-diabetic Chemical Components in Chinese Patent Medicines by HPLC-MS

Determination of 8 Kinds of Chemical Medicines Illegally Added in Traditional Chinese Medicines for Relieving Cough and Asthma by UPLC-Q-TOF

Related Author

FAN Lei-lei

LIU Nai-qiang

LI Zhen-guo

MENG Li

CHEN Wei

OUYANG Ba-si

CHEN Xiao-hu

QIN Jian

Related Institution

No data

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰