HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis

BAI Yan; ZUO Chun-fang; XIE Cai-xia; LEI Jing-wei; LI Lei

doi:10.13422/j.cnki.syfjx.2012.16.075

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HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis

更新时间：2024-09-23

- HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 84-86(2012)
- 作者机构：
  
  河南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.075
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]白雁,左春芳,谢彩侠,雷敬卫,李磊.HPLC-ELSD测定盾叶薯蓣中伪原薯蓣皂苷的含量[J].中国实验方剂学杂志,2012,18(16):84-86.

BAI Yan, ZUO Chun-fang, XIE Cai-xia, et al. HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 84-86.
[1]白雁,左春芳,谢彩侠,雷敬卫,李磊.HPLC-ELSD测定盾叶薯蓣中伪原薯蓣皂苷的含量[J].中国实验方剂学杂志,2012,18(16):84-86. DOI： 10.13422/j.cnki.syfjx.2012.16.075.

BAI Yan, ZUO Chun-fang, XIE Cai-xia, et al. HPLC-ELSD Determination of Pseudoprodioscin in Dioscorea zingiberensis[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 84-86. DOI： 10.13422/j.cnki.syfjx.2012.16.075.

摘要

目的:建立盾叶薯蓣中伪原薯蓣皂苷的含量测定方法。方法:采用高效液相色谱-蒸发光散射检测法（HPLC-ELSD）

Dikma C18色谱柱（4.6 mm×250 mm

5μm）

流动相乙腈-水（30∶70）

流速1.0 mL.min-1;蒸发光散射检测器参数:漂移管温度60℃

气压4.5 kPa。结果:伪原薯蓣皂苷在10～40μg（r=0.999 8）线性关系良好

平均回收率98.7%（RSD 1.6%

n=6）。结论:该方法简便、快速、灵敏、准确

可作为盾叶薯蓣的质量控制方法。

Abstract

Objective:To establish a method for the determination of pseudoprodioscin in Dioscorea zingiberensis.Method:HPLC-ELSD was used in the quantitative analysis with Dikma C18 chromatography column（4.6 mm×250 mm

5 μm） and acetonitrile-water（30∶70） as a mobile phase.The flow rate of mobile was 1.0 mL·min-1;the drifting tube temperature of the detecor was set at 60 ℃.The pressure of pure air was 4.5 kPa.Result: The linear range for pseudoprodioscin was 10-40 μg（r=0.999 8）.The average recovery was 98.7%（RSD 1.6%

n=6）.Conclusion: This method is simple

rapid

sentive

accurate

and can be regarded as the quality control method of D.zingiberensis.

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Related Author

BAI Yan

ZUO Chun-fang

XIE Cai-xia

LEI Jing-wei

LI Lei

FAN Bin1

LIU Hong2

SUN Jian1

Related Institution

School of Pharmaceatical Sciences,Tianjin Medical University

Research Institute of Traditional Chinese Medicine,Heilongjiang University of Chinese Medicine

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