Determination of 6 Amino Acids in Lujiao Buxue Keli by Pre-Column Derivation HPLC

OU Jin-xiu1; GU Zhi-xin1; 3; ZHANG Ni-yu1; XIE Yi4; WANG Shi-qiang4; ZHU Li1; YAN Dong-lan1

doi:10.13422/j.cnki.syfjx.2012.16.078

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Determination of 6 Amino Acids in Lujiao Buxue Keli by Pre-Column Derivation HPLC

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- Determination of 6 Amino Acids in Lujiao Buxue Keli by Pre-Column Derivation HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 93-96(2012)
- 作者机构：
  
  1. 九芝堂股份有限公司
  2. 湖南省中药固体制剂工程技术研究中心
  3. 湖南九芝堂医药科技有限公司
  4. 湖南省中医药研究院
  5. 中国中医科学院中药研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.078
  CLC： R286.0
- Published：2012
- 稿件说明：
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[1]欧金秀,谷陟欣,张妮瑜,谢谊,王实强,朱丽,颜冬兰,于定荣.柱前衍生HPLC同时测定驴胶补血颗粒中6种水解氨基酸[J].中国实验方剂学杂志,2012,18(16):93-96.

OU Jin-xiu1, GU Zhi-xin1, 3, et al. Determination of 6 Amino Acids in Lujiao Buxue Keli by Pre-Column Derivation HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 93-96.
[1]欧金秀,谷陟欣,张妮瑜,谢谊,王实强,朱丽,颜冬兰,于定荣.柱前衍生HPLC同时测定驴胶补血颗粒中6种水解氨基酸[J].中国实验方剂学杂志,2012,18(16):93-96. DOI： 10.13422/j.cnki.syfjx.2012.16.078.

OU Jin-xiu1, GU Zhi-xin1, 3, et al. Determination of 6 Amino Acids in Lujiao Buxue Keli by Pre-Column Derivation HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 93-96. DOI： 10.13422/j.cnki.syfjx.2012.16.078.

摘要

目的:建立柱前衍生反相高效液相色谱法同时测定驴胶补血颗粒中6种水解氨基酸含量的方法。方法:采用Hypersil BDS C18色谱柱（4.6 mm×200 mm

5μm）

异硫氰酸苯酯为柱前衍生剂

梯度洗脱

流速1.0 mL.min-1

检测波长254nm

柱温30℃。结果:L-谷氨酸的线性范围0.169～1.014μg（r=0.999 5）

平均回收率101.43%

RSD 3.20%;L-羟脯氨酸的线性范围0.178～1.068μg（r=0.999 9）

平均回收率101.23%

RSD 3.25%;甘氨酸的线性范围0.381～2.286μg（r=0.999 5）

平均回收率102.87%

RSD 2.32%;L-丙氨酸的线性范围为0.162～0.972μg（r=0.999 5）

平均回收率102.37%

RSD 3.36%;L-精氨酸的线性范围0.118～0.708μg（r=0.999 6）

平均回收率102.73%

RSD 2.41%;L-脯氨酸的线性范围0.253～1.518μg（r=0.999 9）

平均回收率98.69%

RSD 2.34%。结论:建立的方法可靠

可用于驴胶补血颗粒中氨基酸的含量检测。

Abstract

Objective:To develop a HPLC method for determining 6 amino acids in Lujiao Buxue keli.Method: The sample was derived with phenyl isothiocyanate（PITC）.Amino acids were separated on Hypersil BDS C18（4.6 mm×200 mm

5 μm） column at the flow rate of 1.0 mL · min-1

detected at 254 nm.Result: L-Glu had good linearity in the range of 0.169-1.014 μg（r=0.999 5）.The average recovery was 101.43%

RSD was 3.20%;L-Hyp had good linearity in the range of 0.178-1.068 μg（r=0.999 9）.The average recovery was 101.23%

RSD was 3.25%;Gly had good linearity in the range of 0.381-2.286 μg（r=0.999 5）.The average recovery was 102.87%

RSD was 2.32%;L-Ala had good linearity in the range of 0.162-0.972 μg（r=0.999 5）.The average recovery was 102.37%

RSD was 3.36%;L-Arg had good linearity in the ranges of 0.118-0.708 μg（r=0.999 6）.The average recovery was 102.73%

RSD was 2.41%;L-Pro had good linearity in the range of 0.253-1.518 μg（r=0.999 9）.The average recovery was 98.69%

RSD was 2.34%. Conclusion: The method is reliable

which is helpful for the determination of amino acids in Lujiao Buxue keli.

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Related Author

OU Jin-xiu

GU Zhi-xin

ZHANG Ni-yu

XIE Yi

WANG Shi-qiang

ZHU Li

YAN Dong-lan

YANG Wei-ling

Related Institution

Chengdu Baoxiong Traditional Chinese Medicine Co. Ltd.，Pengzhou

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