Determination of Two Chromone Glycosides in Fangfeng Tongsheng Wan by HPLC

NIU De-bin

doi:10.13422/j.cnki.syfjx.2012.16.083

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Determination of Two Chromone Glycosides in Fangfeng Tongsheng Wan by HPLC

更新时间：2024-09-23

- Determination of Two Chromone Glycosides in Fangfeng Tongsheng Wan by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 113-115(2012)
- 作者机构：
  
  息县第二人民医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.083
  CLC： R286.0
- Published：2012
- 稿件说明：
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[1]牛德斌.HPLC测定防风通圣丸中2个色原酮苷类成分含量[J].中国实验方剂学杂志,2012,18(16):113-115.

NIU De-bin. Determination of Two Chromone Glycosides in Fangfeng Tongsheng Wan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 113-115.
[1]牛德斌.HPLC测定防风通圣丸中2个色原酮苷类成分含量[J].中国实验方剂学杂志,2012,18(16):113-115. DOI： 10.13422/j.cnki.syfjx.2012.16.083.

NIU De-bin. Determination of Two Chromone Glycosides in Fangfeng Tongsheng Wan by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 113-115. DOI： 10.13422/j.cnki.syfjx.2012.16.083.

摘要

目的:建立防风通圣丸中2个色原酮苷类成分的含量测定方法。方法:采用HPLC法

对防风通圣丸中的升麻素苷、5-O-甲基维斯阿米醇苷进行定量分析。kromasil C18柱（4.6 mm×250 mm

5μm）;流动相甲醇-乙腈-0.1%磷酸（24∶12∶64）;检测波长254 nm。结果:升麻素苷、5-O-甲基维斯阿米醇苷分别在0.012 5～0.250

0.010 8～0.216μg（r>0.999 9）线性关系良好

平均回收率>96.75%

RSD<1.65%。结论:该研究同时进行升麻素苷、5-O-甲基维斯阿米醇苷的定量分析

可作为防风通圣丸的质量控制标准。

Abstract

Objective:To develop RP-HPLC method for the quantitative determination of two chromones in Fangfeng Tongsheng Wan.Method:The determination was performed on a kromasil C18 column（4.6 mm×250 mm

5 μm） with methanol-acetonitrile-0.1% phosphoric acid（24 ∶ 12 ∶ 64） as the mobile phase at the flow rate of 1.0 mL·min-1.The detection wavelength was set at 254 nm.Result:The calibration curves of prim-O-glucosylcimifugin and 4′-O-glucosyl-5-O-methylvisamminol were linear in the ranges of 0.012 5-0.250 μg and 0.010 8-0.216 μg（r > 0.999 9）

respectively.The average recoveries were over 96.75% with RSD lower 1.65 %.Conclusion:The method is simple

accurate

reproducible

and suitable for simultaneously determination of prim-O-glucosylcimifugin and 4′-O-glucosyl-5-O-methylvisamminol

and can be used for quality control of Fangfeng Tongsheng Wan.

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