GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa

WANG Li1; ZHAO Hua2

doi:10.13422/j.cnki.syfjx.2012.16.086

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GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa

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- GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 16, Pages: 127-132(2012)
- 作者机构：
  
  1. 甘肃农业大学生命科学技术学院
  2. 陕西理工学院生物科学与工程学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.16.086
  CLC： R284.1
- Published：2012
- 稿件说明：
移动端阅览
[1]王莉,赵桦.小丛红景天脂溶性成分及抗菌活性分析[J].中国实验方剂学杂志,2012,18(16):127-132.

WANG Li1, ZHAO Hua2. GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 127-132.
[1]王莉,赵桦.小丛红景天脂溶性成分及抗菌活性分析[J].中国实验方剂学杂志,2012,18(16):127-132. DOI： 10.13422/j.cnki.syfjx.2012.16.086.

WANG Li1, ZHAO Hua2. GC-MS Analysis of Fat-soluble Compositions and Antimicrobial Activity of Rhodiola dumulosa[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(16): 127-132. DOI： 10.13422/j.cnki.syfjx.2012.16.086.

摘要

目的:分析小丛红景天中脂溶性成分组成及体外抗菌抑菌活性。方法:采用索氏提取法

提取陕西太白山产小丛红景天中脂溶性物质

经甲酯化处理后用气相色谱-质谱联用技术（GC-MC）分离和鉴定脂溶性成分的种类及含量。通过体外实验

分析研究小丛红景天脂溶性成分抗菌抑菌活性。结果:脂溶性成分共分离出三个馏分

即:非极性馏分、弱极性馏分和极性馏分。其中非极性馏分成分20种

弱极性馏分成分17种

极性馏分成分12种。抑菌实验表明

小丛红景天脂溶性成分对选定的白假丝酵母菌（CMCC85021）、枯草芽胞杆菌（CMCC63501）、金黄色葡萄球菌（ATCC25925）和大肠埃希氏菌（ATCC25922）四种供试菌株均有很好的抑制作用。结论:小丛红景天中含有一些重要的脂肪酸成分

具有较高的保健营养价值。同时

其脂溶性成分具有很好的抑菌杀菌活性。

Abstract

Objective:To assay antibacterial activity and fat-soluble compositions of Rhodiola dumulosa.Method: The fat-soluble components of R.dumulosa produced in Taibai mountain in Shanxi province were extracted by Soxhlet extraction.The category and content of fat-soluble components were identified and determined by gas chromatography-mass spectrometry（GC-MS） after methyl esterification treatment.The antibacterial activity of fat-soluble components was assayed in vitro.Result: Total 3 kinds of fraction were identified from the fat-soluble components from R.dumulosa.: the non-polarity compounds

weak polarity compounds and polarity compounds.Total 20 kinds of compositions were identified from the non-polarity compounds.Total 17 kinds of compositions were identified from the weak-polarity compounds.Total 12 kinds of compositions were identified from the polarity compounds.The result of antibacterial activity of the fat-soluble component in vitro showed that Escherichia coli

Staphylococcus aureus

Bacillus subtillis and Candida albicans have a better antibacterial activity.Conclusion: It was shown that there were some impotent fat-soluble components in R.dumulosa which have potential value in food nutrition and health care.At the same time

the fat-soluble components have better antibacterial activity.

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