Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides

ZHOU Zhi-wang1; HUANG Hui-lian2; SHAO Feng2; LIU Rong-hua2; ZHAO Hai-ping2; HU Lin-feng2; HE Xiao-ru2; REN Gang2

doi:10.13422/j.cnki.syfjx.2012.18.020

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Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides

更新时间：2024-09-23

- Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 7-10(2012)
- 作者机构：
  
  1. 南昌大学药学系
  2. 江西中医学院现代中药制剂教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.020
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]周志望,黄慧莲,邵峰,刘荣华,赵海平,胡林峰,何小汝,任刚.三脉紫菀总黄酮的提取纯化工艺优选[J].中国实验方剂学杂志,2012,18(18):7-10.

ZHOU Zhi-wang1, HUANG Hui-lian2, SHAO Feng2, et al. Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 7-10.
[1]周志望,黄慧莲,邵峰,刘荣华,赵海平,胡林峰,何小汝,任刚.三脉紫菀总黄酮的提取纯化工艺优选[J].中国实验方剂学杂志,2012,18(18):7-10. DOI： 10.13422/j.cnki.syfjx.2012.18.020.

ZHOU Zhi-wang1, HUANG Hui-lian2, SHAO Feng2, et al. Optimization of Extraction and Purification Technology for Total Flavones from Aster ageratoides[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 7-10. DOI： 10.13422/j.cnki.syfjx.2012.18.020.

摘要

目的:优选三脉紫菀总黄酮的提取、纯化工艺条件。方法:采用正交试验设计

以总黄酮含量为指标

优选三脉紫菀总黄酮的提取工艺条件;以总黄酮静态吸附量和解吸率为考察指标筛选大孔吸附树脂型号

并优化其纯化总黄酮的工艺条件。结果:最佳提取工艺条件为乙醇体积分数60%

浸提温度50℃

浸提3次

料液比1∶10。HPD-500型大孔吸附树脂对三脉紫菀总黄酮有较好的吸附分离性能

以16 mg总黄酮/g树脂的上柱量配制成50 g.L-1的上柱液

上柱后静置1 h

用4 BV水洗除杂

用2 BV 70%乙醇洗脱

收集洗脱液。此工艺条件下

总黄酮纯度76.7%

树脂富集倍数3.83。结论:该优选工艺简便、实用

可为三脉紫菀的工业生产提供参考。

Abstract

Objective:To optimize extraction and purification technology of total flavones from Aster ageratoides.Method:With the content of total flavones as index

extraction technology conditions of total flavones was optimized by orthogonal test;Type of macroporous resin was selected with static adsorption capacity and desorption rate as indexes

and to optimized purification technology of total flavones.Result:Optimum extraction conditions were as follows:soaking extracted 3 times with 10 times the amount of 60% ethanol at 50 ℃.HPD-500 type macro porous resin showed better adsorption and desorption property

optimum purification conditions were as follows:sample solution was prepared with the concentration of 50 g · L-1

subjected to HPD-500 type macroporous resin column chromatography with load ratio of 16 mg total flavones per gram of resin

standing for 1 hour

column was eluted with 4 BV water before being eluted with 2 BV 70% ethanol.Under these conditions

purity of total flavones was 76.7%

which enhanced 3.83 times.Conclusion:This optimized technology was convenient and practical

it could provide a reference for industrial production of A.ageratoides.

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Related Author

ZHOU Zhi-wang

HUANG Hui-lian

SHAO Feng

LIU Rong-hua

ZHAO Hai-ping

HU Lin-feng

HE Xiao-ru

REN Gang

Related Institution

School of Chemistry and Chemical Engineering, Liaocheng University

School of Traditional Chinese Medicine,Guangdong Pharmaceutical College

Department of Pharmacy,Hunan University of Traditional Chinese Medicine

Guangxi University of Chinese Medicine

Affiliated Hospital of Guilin Medical College

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