Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology

SHEN Xiao-zhong1; YANG Fan3; YU Rong-min2

doi:10.13422/j.cnki.syfjx.2012.18.028

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Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology

更新时间：2024-09-23

- Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 34-37(2012)
- 作者机构：
  
  1. 广东食品药品职业学院
  2. 暨南大学药学院
  3. 重庆市食品药品检验所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.028
  CLC： R284
- Published：2012
- 稿件说明：
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[1]沈小钟,杨帆,于荣敏.响应面法优选黄芪提取工艺[J].中国实验方剂学杂志,2012,18(18):34-37.

SHEN Xiao-zhong1, YANG Fan3, YU Rong-min2. Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 34-37.
[1]沈小钟,杨帆,于荣敏.响应面法优选黄芪提取工艺[J].中国实验方剂学杂志,2012,18(18):34-37. DOI： 10.13422/j.cnki.syfjx.2012.18.028.

SHEN Xiao-zhong1, YANG Fan3, YU Rong-min2. Optimization of Extration Technology for Astragaloside Ⅳ from Asrtagalus membranacevs by Response Surface Methodology[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 34-37. DOI： 10.13422/j.cnki.syfjx.2012.18.028.

摘要

目的:响应面法优选黄芪的渗漉提取工艺。方法:采用三因素三水平Box-Behnken试验设计

以乙醇体积分数、流速、乙醇用量为自变量

黄芪甲苷提取率为因变量

HPLC-ELSD测定黄芪甲苷含量

通过对自变量各水平的多元回归及二项式拟合

用响应面法优选乙醇渗漉提取工艺

并进行预测分析。结果:最佳提取工艺条件为30%乙醇

流速1.9 mL.min-1.cm-2

乙醇用量为药材量6.5倍。在此条件下

黄芪甲苷提取率理论值1.50 mg.g-1

实测值1.487 mg.g-1

说明该优选工艺模型拟合度良好。结论:Box-Behnken试验设计可用于黄芪渗漉提取工艺的优选

该优选工艺简便、稳定。

Abstract

Objective:To optimize extraction technology of astragaloside Ⅳ from Astragalus membranaceus by response surface methodology（RSM）.Method:Three-factor and three-level Box-Behnken experimental design was employed with ethanol concentration

flow rate and liquid-solid ratioas independence variables

extraction rate of astragaloside Ⅳ as dependent variable which was determined by HPLC-ELSD

experimental data obtained by multiple regression and binomial fitting of levels from independent variables

ethanol percocation extraction technology was optimized by RSM

and to predictive analysis.Result:Optimum extraction conditions were as follows:30% ethanol as solvent

flow rate 1.9 mL · min-1 · cm-2

liquid-solid ratio 6.5 mL · g-1.Under these conditions

theoretical value of extraction rate of astragaloside Ⅳ 1.50 mg · g-1

but measured value of 1.487 mg · g-1

it showed optimized technology model fitted well.Conclusion:Box-Behnken experimental design could be used for extraction optimization of percocation extraction process for A.membranaceus

optimized technology was simple and stable.

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Related Institution

Hebei Key Laboratory of Traditional Chinese Medicine（TCM） Research on Cardiocerebrovascular Disease，TCM Processing Technology Innovation Center of Hebei Province，College of Pharmacy， Hebei University of Chinese Medicine

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