LI Yun1, MIAO Xiao-lou2, WEI Shu-chang1, et al. Optimization of Ultrafiltration Technology for Polysaccharides from Rheum palmatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 55-58.
LI Yun1, MIAO Xiao-lou2, WEI Shu-chang1, et al. Optimization of Ultrafiltration Technology for Polysaccharides from Rheum palmatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 55-58. DOI: 10.13422/j.cnki.syfjx.2012.18.033.
Optimization of Ultrafiltration Technology for Polysaccharides from Rheum palmatum
Objective:To study on separation and purification feasible process of polysaccharides from R.palmatum by ultrafiltration.Method:Centrifugation conditions were optimized by orthogonal test before ultrafiltration
ultrafiltration technology parameters were optimized by uniform design with yield and concent of polysaccharides as indexes
and ultrafiltration pressure
liquid temperature and liquid concentration were selected as investigated factors.Result:Optimum ultra-filtration process was:centrifuged 12 min with 3 000 r · min-1
membrane with 105 molecular interception
ultrafiltration pressure 0.05-0.09 MPa
liquid temperature 40 ℃
ratio of solid-liquid 1∶10-1∶30
pH 6.0
ultrafiltration membrane was pretreated 10 min with tween-80(5 g·L-1).Conclusion:Optimized ultrafiltration process could obtain higher yield and concent of polysaccharides while compared with non-ultrafiltration.Ultrafiltration technology could be used for purification and enrichment of polysaccharides from R.palmatum with industrial development prospect.
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Related Author
LI Yun
MIAO Xiao-lou
WEI Shu-chang
WU Ping-an
LIU Feng-lin
LI Xiu-juan
Bing-bing ZHANG
Ze-min OU
Related Institution
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences
School of Pharmacy, Jiangxi University of Chinese Medicine
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Ethnic Medicine School, Chengdu University of Traditional Chinese Medicine