Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch

XU Gui-hua1; ZHANG Ji-huan1; QIAO Jian-wei1; SU Rui-qiang1; ZHAO Zhi-quan1; SUN Zong-xi1

doi:10.13422/j.cnki.syfjx.2012.18.036

您当前的位置：

首页 >

文章列表页 >

Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch

更新时间：2024-09-23

- Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 70-73(2012)
- 作者机构：
  
  1. 鲁南制药集团股份有限公司
  2. 中药制药共性技术国家重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.036
  CLC： R284.1
- Published：2012
- 稿件说明：
移动端阅览
[1]徐桂花,张继环,乔建卫,苏瑞强,赵志全,孙宗喜.HPLC波长切换技术同时测定板蓝根中4种活性成分含量[J].中国实验方剂学杂志,2012,18(18):70-73.

XU Gui-hua1, ZHANG Ji-huan1, QIAO Jian-wei1, et al. Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 70-73.
[1]徐桂花,张继环,乔建卫,苏瑞强,赵志全,孙宗喜.HPLC波长切换技术同时测定板蓝根中4种活性成分含量[J].中国实验方剂学杂志,2012,18(18):70-73. DOI： 10.13422/j.cnki.syfjx.2012.18.036.

XU Gui-hua1, ZHANG Ji-huan1, QIAO Jian-wei1, et al. Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 70-73. DOI： 10.13422/j.cnki.syfjx.2012.18.036.

摘要

目的:建立反相高效液相色谱法同时测定板蓝根药材中尿苷、腺苷、鸟苷和表告依春4种活性成分含量的方法

并比较不同时间采收的板蓝根中上述各成分的含量情况。方法:采用Kromasil C18色谱柱（4.6 mm×250 mm

5μm）

流动相为甲醇-0.1%磷酸溶液

梯度洗脱

流速0.7 mL.min-1;双波长切换时间序列采样:0～30 min为254 nm

30～45 min为245 nm;柱温25℃。结果:在上述色谱条件下

测得尿苷、腺苷、鸟苷和表告依春分别在14.80～1 184 ng（r=0.999 9）

14.55～1 164ng（r=0.999 9）

13.75～1 100 ng（r=0.999 9）

12.25～980 ng（r=0.999 9）与峰面积呈良好的线性关系;平均加样回收率:尿苷99.5%（RSD 1.2%）

腺苷98.3%（RSD 1.4%）

鸟苷98.1%（RSD 1.2%）

表告依春99.0%（RSD 0.9%）。不同时间采收板蓝根中4种活性成分的含量是动态变化的。结论:方法操作简便、准确

重复性良好

可用于板蓝根药材的质量控制。

Abstract

Objective:To develop an HPLC method for simultaneous determination of uridine

adenosine

guanosine and epigoitrin in Radix Isatidis

and compare the content variation of the four components of herbs harvested at different time.Method:Four components were separated by Kromasil C18 column（4.6 mm×250 mm

5 μm） with gradient mobile phase of methanol-0.1% phosphoric acid at the flow rate of 0.7 mL ·min-1.The detection wavelength was set at 254 nm（0-30 min） and 245 nm（30-45 min）.The column temperature was maintained at 25 ℃.Result:The calibration curves of uridine

adenosine

guanosine and epigoitrin were in good linearity over the range of 14.80-1 184 ng（r =0.999 9）

14.55-1 164 ng（r=0.999 9）

13.75-1 100 ng（r=0.999 9）

12.25-980 ng（r=0.999 9）

respectively.The average recoveries of the four components were 99.5%（RSD 1.2%）

98.3%（RSD 1.4%）

98.1%（RSD 1.2%）

99.0%（RSD 0.9%）

respectively.The content of four components in Radix Isatidis varied with different harvest time.Conclusion:The method is simple

accurate and reproducible for quality control of Radix Isatidis.

关键词

Keywords

references

Views

162

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Simultaneous Determination of Four Active Components in Radix Isatidis by HPLC with Double-wavelength Switch

Establishment of Quality Control Method in Production of Jinshuibao Capsules

Determination of Content of Uridine,Guanosine and Adenosine in by UPLC

Determination of Uridine, Guanosine and Adenosine Contents in Corydalis Rhizoma from Different Regions and Commercial Grades

Optimization of Extraction Technology for Total Nucleosides from by Orthogomal Test

Related Author

XU Gui-hua

ZHANG Ji-huan

QIAO Jian-wei

SU Rui-qiang

ZHAO Zhi-quan

SUN Zong-xi

Xue-ting ZHANG

Cheng-zan ZHONG

Related Institution

Key Laboratory of Identification and Safety Evaluation of Chinese Medicine, Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Jiangxi Guoyao Co. Ltd.

Shandong University of Traditional Chinese Medicine

The Affiliated Hospital of Shandong University of Traditional Chinese Medicine

He-Run Innovative Medical Technology Development Co.,Ltd,Beijing

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰