Determination of Content of Total Flavonoids from Polygonum perfoliatum

ZHANG Ming1; CHEN Hua-guo1; ZHAO Chao1; ZHOU Xin1; TAN Ji-cang3; XUE Jing3; YANG Shi-lin1

doi:10.13422/j.cnki.syfjx.2012.18.038

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Determination of Content of Total Flavonoids from Polygonum perfoliatum

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- Determination of Content of Total Flavonoids from Polygonum perfoliatum
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 77-80(2012)
- 作者机构：
  
  1. 贵州师范大学天然药物质量控制研究中心
  2. 贵州省山地环境信息系统与生态环境保护重点实验室
  3. 贵州远程制药有限责任公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.038
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]张明,陈华国,赵超,周欣,谭济苍,薛静,杨世林.杠板归中总黄酮的含量测定[J].中国实验方剂学杂志,2012,18(18):77-80.

ZHANG Ming1, CHEN Hua-guo1, ZHAO Chao1, et al. Determination of Content of Total Flavonoids from Polygonum perfoliatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80.
[1]张明,陈华国,赵超,周欣,谭济苍,薛静,杨世林.杠板归中总黄酮的含量测定[J].中国实验方剂学杂志,2012,18(18):77-80. DOI： 10.13422/j.cnki.syfjx.2012.18.038.

ZHANG Ming1, CHEN Hua-guo1, ZHAO Chao1, et al. Determination of Content of Total Flavonoids from Polygonum perfoliatum[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 77-80. DOI： 10.13422/j.cnki.syfjx.2012.18.038.

摘要

目的:建立杠板归中总黄酮的含量测定方法。方法:以芦丁为对照品

以0.1 mol.L-1AlCl3溶液、1 mol.L-1NaAc溶液为显色剂

检测波长413 nm。结果:芦丁吸光度与其浓度在6.24～21.84μg线性良好（r=0.999 8）

总黄酮回归方程为Y=38.183X-0.001;平均加样回收率为97.4%

RSD 2.2%。结论:方法简便、稳定

可用于杠板归中总黄酮的含量测定。

Abstract

Objective:To establish a method for determination of total flavonoids in Polygonum perfoliatum.Method:Total flavoaoids were determined by UV-Vis spectrophotometer with rutiu as reference substance and 0.1 mol · L-1 AlCl3

1 mol · L-1 NaAc as chromogenic agent;the detection wavelength was at 413 nm.Result:A good linear relationship was achieved over the concentration range of 6.24-21.84 μg（r =0.999 8） for Rutin.The regression equation of total flavonoids was Y=38.183X-0.001

with an average recovery of 97.4%

RSD 2.2%.Conclusion:The method is simple and stable

and it is suitable for the content determination of total flavonoids from P.perfoliatum.

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Related Author

ZHANG Ming

CHEN Hua-guo

ZHAO Chao

ZHOU Xin

TAN Ji-cang

XUE Jing

YANG Shi-lin

LIANG Bao-jun

Related Institution

Affiliated Hospital of Guilin Medical College

Guilin Medical University

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

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