ZHANG Zheng-guang1, ZHU Ling-ying1, QIAN Shi-hui1. HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of Acanthopanax gracilistylus[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 81-84.
ZHANG Zheng-guang1, ZHU Ling-ying1, QIAN Shi-hui1. HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of Acanthopanax gracilistylus[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 81-84. DOI: 10.13422/j.cnki.syfjx.2012.18.039.
HPLC-ELSD Determination of Content of Oleanane-type Triterpenoidal Saponins in the Fruits of Acanthopanax gracilistylus
摘要
目的:建立HPLC-ELSD法同时测定细柱五加果实中的竹节参苷Ⅳa和3β-([O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid含量的方法。方法:采用反相高效液相色谱-蒸发光散射检测法。色谱条件AlltimaTM-C18色谱柱(4.6mm×250 mm
Objective:To establish an HPLC-ELSD method for the simultaneous determination of the content of chikusetsusaponin Ⅳa and 3β-([O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.Method:The RP-HPLC-ELSD method was used.Chromatographic conditions:AlltimaTM-C18 chromatographic column(4.6 mm×250 mm
5 μm);acetonitrile(A)-0.5% acetic acid water(B) as mobile phase;column temperature at 35 ℃.ELSD detector conditions:drift tube temperature:106 ℃;carrier gas(nitrogen) flow rate:2.9 L · min-1;amplification factor:1;impactor:close.Result:The calibration curves showed good linear relationship in the range from 0.535 to 8.560 μg(r=0.996 5) for chikusetsusaponin Ⅳa and 0.515 to 8.240 μg(r=0.998 7) for 3β-([O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid.The average recovery rates(n=6) were 98.39% and 98.97%
and RSDs were 1.50% and 1.59%.Conclusion:This method is accurate
sensitive and can be used to determine the content of chikusetsusaponin Ⅳa and 3β-([O-β-D-glucopyranuronosyl]oxy)-olean-12-ene-28-olc acid in the fruits of Acanthopanax gracilistylus.