Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC

ZHAO Yan-li; HUANG Yi-qi; HU Shan-mei; YANG Hui

doi:10.13422/j.cnki.syfjx.2012.18.042

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Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC

更新时间：2024-09-23

- Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 95-97(2012)
- 作者机构：
  
  厦门市医药研究所厦门市天然药物研究与开发重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.042
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]赵艳丽,黄亦琦,胡珊梅,杨辉.HPLC测定全蚕粉中槲皮素和山奈素的含量[J].中国实验方剂学杂志,2012,18(18):95-97.

ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 95-97.
[1]赵艳丽,黄亦琦,胡珊梅,杨辉.HPLC测定全蚕粉中槲皮素和山奈素的含量[J].中国实验方剂学杂志,2012,18(18):95-97. DOI： 10.13422/j.cnki.syfjx.2012.18.042.

ZHAO Yan-li, HUANG Yi-qi, HU Shan-mei, et al. Content Determination of Quercetin and Kaempferol in Silkworm Powder by RP-HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 95-97. DOI： 10.13422/j.cnki.syfjx.2012.18.042.

摘要

目的:建立全蚕粉中槲皮素、山奈素的含量测定方法。方法:采用反相高效液相色谱法（RP-HPLC）

色谱柱为KromasiL-C18柱（4.6 mm×250 mm

5μm）

柱温为室温

流动相甲醇-0.4%磷酸（50∶50）

流速1.0 mL.min-1

检测波长360 nm

进样量10μL。结果:槲皮素和山奈素得到良好分离

分别在0.012 5～0.400 0μg（r=0.999 8）

0.014 0～0.448 0μg（r=0.999 9）呈良好的线性关系

平均回收率分别为97.53%（RSD 1.9%）

100.3%（RSD 1.0%）。结论:该方法快速准确

可满足定量分析的需要。

Abstract

Objective:To establish assaying methods for the determination of quercetin and kaempferol in Silkworm Powder.Method:The content of quercetin and kaempferol was determined by RP-HPLC

using a KromasiL-C18 column（4.6 mm×250 mm

5 μm）.The column temperature was fixed on room temperature.Methanol-0.4% phosphoric acid（50∶50） was used as mobile phase

flow-rate was 1.0 mL · min-1.The detecting wavelength was set at 360 nm.Result:The linear range were 0.012 5-0.4 μg（r=0.999 8） and 0.014-0.448 μg（r=0.999 9）.The average recoveries were 97.53% and 100.3%

with the RSD of 1.9% and 1.0%.Conclusion:The method established is rapid and accurate

which could be used for quality control of Silkworm Powder.

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Related Institution

Guangxi Key Laboratory of Traditional Chinese Medicine Quality Standards

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