Determination of Content of Eldrin,Quertetin and Kaempferol in Impatiens balsamina by HPLC

TIAN Zhen-kun1; GU Yuan-yuan2; SONG Cheng-gui1; DAI Jin-hong1; WANG Jian-ming2

doi:10.13422/j.cnki.syfjx.2012.18.044

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Determination of Content of Eldrin,Quertetin and Kaempferol in Impatiens balsamina by HPLC

更新时间：2024-09-23

- Determination of Content of Eldrin,Quertetin and Kaempferol in Impatiens balsamina by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 106-108(2012)
- 作者机构：
  
  1. 黑龙江中医药大学药学院
  2. 黑龙江中医药大学中医药研究院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.044
  CLC： R284.1
- Published：2012
- 稿件说明：
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[1]田振坤,顾媛媛,宋成贵,代金红,王建明.HPLC测定凤仙透骨草中的芦丁、槲皮素和山奈酚的含量[J].中国实验方剂学杂志,2012,18(18):106-108.

TIAN Zhen-kun1, GU Yuan-yuan2, SONG Cheng-gui1, et al. Determination of Content of Eldrin,Quertetin and Kaempferol in Impatiens balsamina by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 106-108.
[1]田振坤,顾媛媛,宋成贵,代金红,王建明.HPLC测定凤仙透骨草中的芦丁、槲皮素和山奈酚的含量[J].中国实验方剂学杂志,2012,18(18):106-108. DOI： 10.13422/j.cnki.syfjx.2012.18.044.

TIAN Zhen-kun1, GU Yuan-yuan2, SONG Cheng-gui1, et al. Determination of Content of Eldrin,Quertetin and Kaempferol in Impatiens balsamina by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 106-108. DOI： 10.13422/j.cnki.syfjx.2012.18.044.

摘要

目的:建立凤仙透骨草中的芦丁、槲皮素和山奈酚的HPLC含量测定方法。方法:采用Diamonsil C18（4.6 mm×250 mm

5μm）色谱柱;以乙睛-0.1%冰乙酸为流动相进行线性梯度洗脱

流速1.0 mL.min-1

柱温30℃

检测波长260 nm。结果:芦丁、槲皮素和山奈酚与其他组份的色谱峰均得到基线分离

加样回收率平均值分别为98.89%

98.39%

98.48%

RSD分别为2.05%

1.51%

1.89%。结论:该方法快速、准确、重复性好

可作为凤仙透骨草质量控制的有效方法。

Abstract

Objective:To establish a HPLC method for the determination of eldrin

quertetin and kaempferol in Impatiens balsamina.Method:The determination was performed on a Diamonsil C18 column（4.6 mm×250 mm

5 μm）

with a gredient mobile phase of acetonitrile-0.1% glacial acetic acid;the flow rate was1.0 mL·min-1;column temperature was 30 ℃;the detection wavelength was at 260 nm.Result:The chromatographic peaks of eldrin

quertetin and kaempferol with other components were completely separated.The average recovery was 98.71%

98.96%

98.89%

RSD 2.13%

2.34%

2.38%.Conclusion:The method was rapid and accurate for quality control of I.balsamina

and laid foundations for further research and development.

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Related Author

TIAN Zhen-kun

GU Yuan-yuan

SONG Cheng-gui

DAI Jin-hong

WANG Jian-ming

Hong-xing MA

Long-fei LIN

Yu-ling LIU

Related Institution

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

Jilin Huakang Pharmaceutical Co. Ltd.

The First Clinical Medical College of Guangzhou University of Chinese Medicine

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