Determination of Hydroxysafflor Yellow A,Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC

NI Lin; YANG Xi

doi:10.13422/j.cnki.syfjx.2012.18.045

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Determination of Hydroxysafflor Yellow A,Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC

更新时间：2024-09-23

- Determination of Hydroxysafflor Yellow A,Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 109-112(2012)
- 作者机构：
  
  甘肃省食品药品检验所甘肃省中药品质与安全评价工程技术研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.045
  CLC： R286.0
- Published：2012
- 稿件说明：
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[1]倪琳,杨锡.HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量[J].中国实验方剂学杂志,2012,18(18):109-112.

NI Lin, YANG Xi. Determination of Hydroxysafflor Yellow A,Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 109-112.
[1]倪琳,杨锡.HPLC测定五味渣驯丸中羟基红花黄色素A、没食子酸和马兜铃酸A的含量[J].中国实验方剂学杂志,2012,18(18):109-112. DOI： 10.13422/j.cnki.syfjx.2012.18.045.

NI Lin, YANG Xi. Determination of Hydroxysafflor Yellow A,Gallic Acid and Aristolochic Acid A in Wuwei Zhaxun Pill by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 109-112. DOI： 10.13422/j.cnki.syfjx.2012.18.045.

摘要

目的:建立五味渣驯丸中羟基红花黄色素A、没食子酸、马兜铃酸A的含量测定方法。方法:采用HPLC对制剂中主要成分羟基红花黄色素A、没食子酸、马兜铃酸A进行定量分析。CAPCELL PAK C18色谱柱（4.6 mm×250 mm

5μm）

甲醇-0.4%磷酸（30∶70）、甲醇-0.2%磷酸（7∶93）、甲醇-0.05%冰醋酸（79∶21）为流动相

流速1.0 mL.min-1

检测波长为403

273

390 nm。结果:羟基红花黄色素A、没食子酸、马兜铃酸A的线性范围分别为0.116～1.740μg（r=0.999 97）

0.067～0.673μg（r=0.999 95）

0.024～0.247μg（r=0.999 99）;平均回收率分别为98.62%（RSD 2.15%）

100.01%（RSD 2.03%）

96.83%（RSD 1.74%）。结论:本方法简便、结果准确可靠

可有效控制制剂质量。

Abstract

Objective:To establish an method for determination of hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill.Method:The hydroxysafflor yellow A

gallic acid and aristolochic acid A in Wuwei Zhaxun pill was determined by HPLC.CAPCELL PAK C18（4.6 mm×250 mm

5 μm）column was used and detection wavelength was at 403

273

390 nm.The mobile phase was methanol-0.4% phosphoric acid（30∶70）

methanol-0.2%phosphoric acid（7∶93）

methanol-0.05% glacial acetic acid（79∶21）

with the flow rate of 1.0 mL · min-1.Result:The calibration curves of hydroxysafflor yellow A

gallic acid and aristolochic acid A were in a good linearity over the ranges of 0.116-1.740 μg（r=0.999 97）

0.067-0.673 μg（r=0.999 95）

0.024-0.247 μg（r=0.999 99）

respectively.The average recoveries were 98.62%（RSD 2.15%）

100.01%（RSD 2.03%）

96.83%（RSD 1.74%）.Conclusion:The method is simple

accurate and can be used for the control.

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Related Author

NI Lin

YANG Xi

LI Yan-fang1

XIA Quan2

XU Feng-qing1

LIU Jin-qi1

DING Jia-xin

ZHANG Qiu-hai

Related Institution

Guiyang Collage of Traditional Chinese Medicine

Guiyang College of Traditional Chinese Medicine

School of Life Sciences，Beijing University of Chinese Medicine

Affiliated Hospital of Nanjing University of Chinese Medicine/Jiangsu Province Hospital of Chinese Medicine

The First Clinical Medical College，Nanjing University of Chinese Medicine

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