CHEN Qiang. Determination of Quercetin in Ilex kudingcha by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 113-114.
CHEN Qiang. Determination of Quercetin in Ilex kudingcha by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 113-114. DOI: 10.13422/j.cnki.syfjx.2012.18.046.
Determination of Quercetin in Ilex kudingcha by HPLC
Objective:The content ofquercetin in Ilex kudingcha was determined by HPLC.Method:The separation was performed on a Kromasil C18 column(4.6 mm×250 mm
5 μm) with methanol-0.4% phosphoric acid(50 ∶ 50) as mobile phase
detected at 371 nm and 30 ℃.Result:Quercetinwas linear in the range of 5.98-47.84 g · mL-1(r=0.999 7).Conclusion:The content ofquercetin in Ilex kudingcha could be determined by HPLC precisely.