Effect of Gegen Decoction on Primary Dysmenorrhea with Cold-damp Coagulation Induced by Oxytocin and Cold Simulating in Mice

YUE Xin-yi; CHAI Cheng-zhi; KOU Jun-ping; YU Bo-yang

doi:10.13422/j.cnki.syfjx.2012.18.061

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Effect of Gegen Decoction on Primary Dysmenorrhea with Cold-damp Coagulation Induced by Oxytocin and Cold Simulating in Mice

更新时间：2024-09-23

- Effect of Gegen Decoction on Primary Dysmenorrhea with Cold-damp Coagulation Induced by Oxytocin and Cold Simulating in Mice
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 174-177(2012)
- 作者机构：
  
  中国药科大学中药复方研究室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.061
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]乐心逸,柴程芝,寇俊萍,余伯阳.葛根汤对缩宫素复合寒冷刺激诱导的寒湿凝滞型原发性痛经小鼠模型的影响[J].中国实验方剂学杂志,2012,18(18):174-177.

YUE Xin-yi, CHAI Cheng-zhi, KOU Jun-ping, et al. Effect of Gegen Decoction on Primary Dysmenorrhea with Cold-damp Coagulation Induced by Oxytocin and Cold Simulating in Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 174-177.
[1]乐心逸,柴程芝,寇俊萍,余伯阳.葛根汤对缩宫素复合寒冷刺激诱导的寒湿凝滞型原发性痛经小鼠模型的影响[J].中国实验方剂学杂志,2012,18(18):174-177. DOI： 10.13422/j.cnki.syfjx.2012.18.061.

YUE Xin-yi, CHAI Cheng-zhi, KOU Jun-ping, et al. Effect of Gegen Decoction on Primary Dysmenorrhea with Cold-damp Coagulation Induced by Oxytocin and Cold Simulating in Mice[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 174-177. DOI： 10.13422/j.cnki.syfjx.2012.18.061.

摘要

目的:探讨葛根汤对寒湿凝滞型原发性痛经小鼠模型的影响和可能的作用机制。方法:将70只雌性ICR小鼠随机分为空白组、模型组、布洛芬组、桂枝茯苓胶囊组和葛根汤低、中、高剂量组

每组10只。连续3 d ip雌激素使小鼠子宫同步化并进行寒冷刺激

第4天ip缩宫素注射液造模

观察30 min内小鼠扭体次数

并测定子宫匀浆液内钙离子（Ca2+）和一氧化氮（NO）的水平。造模的同时

布洛芬、桂枝茯苓胶囊、葛根汤低、中、高组分别按0.12

1.12

3.5

7.0

14.0 g.kg-1.d-1的剂量ig给药。结果:葛根汤低、中、高剂量均能够有效地抑制缩宫素复合寒冷刺激诱导的扭体反应

抑制率分别为81.03%

84.14%

90.15%;能够提高子宫匀浆液中NO水平、降低Ca2+水平（P<0.001）

与目前治疗原发性痛经的一线药物布洛芬和桂枝茯苓胶囊的药效相当。结论:葛根汤能有效缓解缩宫素复合寒冷刺激诱导的子宫平滑肌痉挛

其作用机制可能与影响子宫组织中Ca2+和NO水平有关。

Abstract

Objective:To investigate the influence of Gegen decoction（GGD） and the potential mechanism on primary dysmenorrhea with cold-damp coagulation induced byoxytocin and cold simulating.Method:Seventy female ICR mice were randomly divided into seven groups:normal group

model group

buprofen group

Guizhi Fuling capsule group

low-dose GGD group

middle-dose GGD group and high-dose GGD group.Subcutaneous injection of estrogen and cold stimulation were used for 3 consecutive days

and peritoneal injection of oxytocin on the 4th day to construct primary dysmenorrhea model with cold-damp coagulation

the writhing response and the levels of nitric oxide（NO） and calcium ion（Ca2+） in mice uterine tissue were investigated.Meanwhile

ibuprofen group received ibuprofen（0.12 g · kg-1）

GFC group received Guizhi Fuling Capsule（1.12 g · kg-1）

GGD group received GGD（low-dose

3.5 g · kg-1;middle-dose

7.0 g · kg-1;high-dose

14.0 g · kg-1）.Result:GGD could inhibit the writhing response effectively（the inhibition rate was 81.03%

84.14%

90.15% at low

middle and high-dose

respectively） and increase NO levels and reduce Ca2+ concentration in uterine tissue of mice（P<0.001）.The efficacy of GGD was equal to the positive drug ibuprofen and Guizhi Fuling capsule.Conclusion:GGD could effectively alleviate the spasm of uterine smooth induced by oxytocin and cold.The mechanism might be relative to regulation of uterine NO and Ca2+ levels.

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YUE Xin-yi

CHAI Cheng-zhi

KOU Jun-ping

YU Bo-yang

WANG Ting

MA Yuncheng

SHI Yuanyuan

LIU Zhen

Related Institution

School of Life Sciences，Beijing University of Chinese Medicine

School of Chinese Materia Medica， Beijing University of Chinese Medicine

Beijing Research Institute of Traditional Medicine， Beijing University of Chinese Medicine

School of Traditional Chinese Medicine，Hunan University of Chinese Medicine

Key Laboratory of Modern Chinese Medicine Preparation of Ministry of Education， Jiangxi University of Chinese Medicine

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