Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats

SHANG Li-zhi; WANG Fu; MIAO Xiao-ling; ZHANG Hui-na; GAN Chen-fei

doi:10.13422/j.cnki.syfjx.2012.18.066

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Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats

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- Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 194-198(2012)
- 作者机构：
  
  河南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.066
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]尚立芝,王付,苗小玲,张慧娜,甘陈菲.四逆散加味抗大鼠肝纤维化作用机制[J].中国实验方剂学杂志,2012,18(18):194-198.

SHANG Li-zhi, WANG Fu, MIAO Xiao-ling, et al. Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 194-198.
[1]尚立芝,王付,苗小玲,张慧娜,甘陈菲.四逆散加味抗大鼠肝纤维化作用机制[J].中国实验方剂学杂志,2012,18(18):194-198. DOI： 10.13422/j.cnki.syfjx.2012.18.066.

SHANG Li-zhi, WANG Fu, MIAO Xiao-ling, et al. Effects and Mechanism of Supplemental Sini San on Hepatic Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 194-198. DOI： 10.13422/j.cnki.syfjx.2012.18.066.

摘要

目的:探讨四逆散加味对肝纤维化的防治作用机制。方法:80只Wister大鼠随机分为8组:正常对照组、病理模型组、四逆散组、四逆散加味高、中、低剂量组、四逆散预防组。除正常对照组外

其余各组均采用猪血清ip诱发肝纤维化

0.5 mL/只

2次/周

连续10周

5周后即可形成肝纤维化。预防组于造模同时给药（以四逆散加味7 g.kg-1）

各治疗组于造模第6周给药

连续4周。四逆散组4 g.kg-1

四逆散加味高、中、低剂量组（14

3.5 g.kg-1）。采用酸性水解法检测肝组织羟脯氨酸（HYP）含量;放射免疫法（RIA）检测肝组织白介素-1（IL-1）和肿瘤坏死因子-α（TNF-α）含量;原位杂交、免疫组化法分别检测肝组织转化生长因子-β1（TGF-β1）和α-平滑肌肌动蛋白（α-SMA）mRNA与蛋白表达。结果:与模型组比较

四逆散加味中剂量组大鼠肝组织羟脯氨酸（478.32±42.35）vs（327.09±39.41）μg.L-1

P<0.01;IL-1（0.58±0.89）vs（0.35±0.47）μg.L-1（P<0.05）;TNF-α（4.82±0.49）vs（4.21±0.45）μg.L-1（P<0.05）

含量均显著降低

TGF-β1 mRNA（9.92±2.57）vs（5.27±1.39）

（P<0.01）和α-SMA mRNA（12.87±0.39）vs（6.33±0.72）

（P<0.01）及其蛋白表达显著减弱（均P<0.01）。结论:四逆散加味有明显的抗肝纤维化作用

其机制可能与抑制TGF-β1和α-SMA基因表达有关。

Abstract

Objective:To investigate the protective effects and its mechanism of Supplemental Sini San（SNS） on hepatic fibrosis in rats.Method:The immunohepatic fibrosis model was induced by intraperitoneal injection of porcine serum.Eighty Wister rats were divided into eight groups:normal control

model control

Sini San Jiawei（SNSJW） high-dose

mediate-dose and low-dose

prevention and SNS group.Except the normal control groups

all the other groups were injected with pig’s serum without inactivation into abdominal cavity

twice per week and 0.5 mL each time

persisting for 10 weeks.The prevention groups were orally given with the medicines（SNSJW 7 g · kg-1

10 weeks）at the same time models wasduplicated

the other treatment groups were dealed with just as the prevention groups 6 weeks later

lasted for 4 weeks.The dose was following:SNS group 4 g · kg-1

SNSJW high-dose group 14 g · kg-1

mediate-dose group 7 g · kg-1

low-dose group 3.5 g · kg-1.The changes of hepatic fibrosis were detected.The content of liver tissue interleukin-1（IL-1） and tumor necrosis factor-alpha（TNF-α）were tested by RIA.In situ hybridization assay and immunohistochemical S-P method were used to detect the expression of transforming growth factor-β1（TGF-β1） mRNA and α-smoth muscle actin（α-SMA） mRNA and there protein in hepatic fibrosis tissues.Result:Compared with model group in SNSJW mediate-dose group

the content of HYP

IL-1

TNF-α in liver tissue was depressed significantly

the expression of TGF-β1 and α-SMA genes was significantly lower in SNSJW mediate-dose group.Conclusion:Supplemental SNS has an action on hepatic fibrosis in rats.The mechanism is related with its inhibiting the expression of TGF-β1 and α-SMA genes in rats liver.

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SHANG Li-zhi

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Related Institution

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