Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana

BAI Zhen-fang1; LU Jing-kun2; WANG Xiao-qin2; LIU Yong1

doi:10.13422/j.cnki.syfjx.2012.18.076

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Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana

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- Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 232-235(2012)
- 作者机构：
  
  1. 北京中医药大学中药学院
  2. 内蒙古医学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.076
  CLC： R285.5
- Published：2012
- 稿件说明：
移动端阅览
[1]白贞芳,陆景坤,王晓琴,刘勇.弯管列当提取物的抗氧化活性[J].中国实验方剂学杂志,2012,18(18):232-235.

BAI Zhen-fang1, LU Jing-kun2, WANG Xiao-qin2, et al. Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 232-235.
[1]白贞芳,陆景坤,王晓琴,刘勇.弯管列当提取物的抗氧化活性[J].中国实验方剂学杂志,2012,18(18):232-235. DOI： 10.13422/j.cnki.syfjx.2012.18.076.

BAI Zhen-fang1, LU Jing-kun2, WANG Xiao-qin2, et al. Anti-oxidant Properties of Extracts from Orobanche cernua var.cumana[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 232-235. DOI： 10.13422/j.cnki.syfjx.2012.18.076.

摘要

目的:研究弯管列当不同提取部位对DPPH（1

1-二苯基苦基苯肼）自由基的清除能力及对H2O2诱导的人胚肾细胞系HEK-293细胞氧化损伤的保护作用。方法:弯管列当70%乙醇粗提物

依次用乙酸乙酯、正丁醇萃取后

得到乙酸乙酯提取部位（gg1）、正丁醇提取部位（gg2）和水部位（gg3）。用清除DPPH自由基法测试gg1

gg2

gg3的抗氧化活性。用H2O2建立人胚肾细胞系HEK-293细胞氧化损伤模型

用MTT法检测细胞活力

观察gg1

gg2

gg3对氧化受损细胞活力的影响。结果:DPPH实验的IC50从小到大依次为:gg1（0.042 9 g.L-1）<gg2（0.059 9 g.L-1）<VC（0.071 8 g.L-1）<gg3（0.330 3 g.L-1）

表明gg1

gg2具有较强的抗氧化活性。研究结果亦表明弯管列当的gg1

gg2

gg3均能明显提高受损伤HEK-293细胞的活力

表现出抗氧化活性。结论:弯管列当以上各提取部位均具有一定的清除自由基、抗氧化的活性。

Abstract

Objective:To investigate 1

1-diphenyl-2-picrylhydrazy1（DPPH） free radical scavenging activities of extracts from Orobanche cernua var.cumana.Method:The 70% ethanol extracts from O.cernua var.cumana was extracted successively with ethyl acetate and n-butanol.The three fractions of ethyl acetate

n-butanol

and water were tested using assays of the scavenging effects on DPPH.The model of oxidative damage of human embryonic kidney cell line HEK-293 cells was induced by H2O2

MTT assay was used to evaluate the cell viability.Result:The IC50 in DPPH test were in the following orders:ethyl acetate（0.042 9 g · L-1）<n-butanol（0.059 9 g · L-1）<VC（0.071 8 g · L-1）<water（0.330 3 g · L-1）.The ethyl acetate fraction showed the strongest scavenging activity to DPPH free radicals.The three fractions of ethyl acetate

n-butanol and water could significantly improve the survival rate of injured HEK-293 cells

showing the antioxidant activities.Conclusion:These results demonstrate that the extracts from O.cernua var.cumana have some anti-oxidant and free radical scavenging activities.

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