Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats

XIE Gao-yu1; YANG Bing-yao2; OU Jiang-qin3; LIN Chang1; CHEN Yun-zhi1; WANG Jian-ke1; YANG Chang-fu

doi:10.13422/j.cnki.syfjx.2012.18.077

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Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats

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- Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 236-239(2012)
- 作者机构：
  
  1. 贵阳中医学院
  2. 贵州省凯里市第一人民医院
  3. 贵阳中医学院第一附属医院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.077
  CLC： R285.5
- Published：2012
- 稿件说明：
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[1]谢高宇,杨冰瑶,欧江琴,林昶,陈云志,王建科,杨长福.加减补肺汤对肺纤维化大鼠的抗氧化作用[J].中国实验方剂学杂志,2012,18(18):236-239.

XIE Gao-yu1, YANG Bing-yao2, OU Jiang-qin3, et al. Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 236-239.
[1]谢高宇,杨冰瑶,欧江琴,林昶,陈云志,王建科,杨长福.加减补肺汤对肺纤维化大鼠的抗氧化作用[J].中国实验方剂学杂志,2012,18(18):236-239. DOI： 10.13422/j.cnki.syfjx.2012.18.077.

XIE Gao-yu1, YANG Bing-yao2, OU Jiang-qin3, et al. Antioxidant Effects of Addition and Subtraction Bufei Decoction on Pulmonary Fibrosis in Rats[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 236-239. DOI： 10.13422/j.cnki.syfjx.2012.18.077.

摘要

目的:探讨加减补肺汤对肺纤维化（PF）大鼠抗氧化作用。方法:SD雄性大鼠40只

随机分为假手术组、模型组、加减补肺汤组

采用一次性气管滴注博来霉素复制PF模型（1 mg/只）

假手术组滴注等量的生理盐水。术后2 d加减补肺汤组ig中药水煎液10 mL.kg-1（含生药16 g.kg-1）

模型组和假手术组ig等量生理盐水

28 d处死动物

取血和肺脏

测定血清超氧化物歧化酶（SOD）活性、丙二醛（MDA）和肺组织中羟脯氨酸（Hyp）的含量

实时荧光PCR测定肺组织中细胞外超氧化物歧化酶（ECSOD）mRNA的表达。结果:加减补肺汤明显改善肺纤维化大鼠的生存状态

降低肺组织中Hyp的含量

降低血清MDA含量

增加ECSOD mRNA的表达。结论:加减补肺汤能提高肺纤维化大鼠生存状态

改善纤维化指标

其机制可能与降低血清中MDA含量

上调ECSOD mRNA表达有关。

Abstract

Objective:To investigate the antioxidant effects of addition and subtraction Bufei decoction（ASBD） on pulmonary fibrosis（PF） in rats.Method:Forty male Sprague-Dawley rats were divided randomly into sham operation group

model group

ASBD group.The PF model was reproduced by intratracheal injection of bleomycin.ASBD group was given traditional Chinese medicine decoction（ig

2 times to clinic dose

16 g · kg-1） starting from 2 days after operation.The sham operation group and model group were given the same amount of normal saline.The blood and lung tissue of rats were took on the 14 d and 28 d.The superoxide dismutase（SOD） activity and malondialdehyde（MDA） content in serum were measured.The content of hydroxyproline in lung tissue was detected.The mRNA of extracellular SOD（ECSOD） was measured by real-time PCR.Result:ASBD significantly improved the survival state of PF rats

reduced content of hydroxyproline in the lung tissue

decreased the level of MDA in the serum

improved the expression of ECSOD mRNA.Conclusions:ASBD could improve the survival rate of PF rats

and the fibrosis index.The mechanism might be related to decrease in the level of serum MDA and up-regulation of the expression of ECSOD mRNA.

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XIE Gao-yu

YANG Bing-yao

OU Jiang-qin

LIN Chang

CHEN Yun-zhi

WANG Jian-ke

YANG Chang-fu

HUANG Lijin

Related Institution

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