TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte

ZHANG Chang-hua1; HU Xian-hong1; ZHANG Zhong-wei2; LIU Yu-hui1; YU Ri-yue1; XU Guo-liang2

doi:10.13422/j.cnki.syfjx.2012.18.091

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TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte

更新时间：2024-09-23

- TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 18, Issue 18, Pages: 171-174(2012)
- 作者机构：
  
  1. 江西中医学院药学院
  2. 中山大学中山医学院药理学教研室
  3. 江西中医学院现代中药制剂教育部重点实验室
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2012.18.091
  CLC： R-332
- Published：2012
- 稿件说明：
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[1]章常华,胡宪红,张忠伟,刘玉晖,余日跃,徐国良.肿瘤坏死因子-α诱导3T3-L1脂肪细胞胰岛素抵抗模型的建立[J].中国实验方剂学杂志,2012,18(18):171-174.

ZHANG Chang-hua1, HU Xian-hong1, ZHANG Zhong-wei2, et al. TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 171-174.
[1]章常华,胡宪红,张忠伟,刘玉晖,余日跃,徐国良.肿瘤坏死因子-α诱导3T3-L1脂肪细胞胰岛素抵抗模型的建立[J].中国实验方剂学杂志,2012,18(18):171-174. DOI： 10.13422/j.cnki.syfjx.2012.18.091.

ZHANG Chang-hua1, HU Xian-hong1, ZHANG Zhong-wei2, et al. TNF-α Induced Insulin Resistance Model in 3T3-L1 Adipocyte[J]. Chinese journal of experimental traditional medical formulae, 2012, 18(18): 171-174. DOI： 10.13422/j.cnki.syfjx.2012.18.091.

摘要

目的:应用肿瘤坏死因子α（TNF-α）诱导3T3-L1脂肪细胞

探讨建立可靠胰岛素抵抗（IR）细胞模型的方法。方法:3T3-L1前脂肪细胞经3-异丁基-1-甲基黄嘌呤、地塞米松、胰岛素诱导分化成3T3-L1脂肪细胞

将其与20

5μg.L-1TNF-α共孵育

100 nmol.L-1胰岛素作用30 min刺激脂肪细胞糖转运。以葡萄糖氧化酶法测定培养基上清液葡萄糖含量

观察TNF-α对脂肪细胞糖摄取的影响

鉴定IR模型。结果:TNF-α抑制胰岛素诱导前、后的脂肪细胞糖转运

抑制作用呈剂量依赖性

其中20μg.L-1TNF-α的抑制率分别为79.2%和81.4%（P<0.05）。结论:肿瘤坏死因子α可诱导3T3-L1脂肪细胞产生IR

这种细胞模型简便、可靠。

Abstract

Objective:To study the effect of tumor necrosis factor α（TNF-α） on glucose consumption in 3T3-L1 adipocyte and find the method of establishing a cell insulin resistance model.Method:The 3T3-L1 preadipocytes were differentiated to 3T3-L1 adipocytes with 1-methyl-3-isobuthylxanthine

dexamethasone and insulin.3T3-L1 adipocytes were exposed to 20

5 μg · L-1 TNF-α for 96 h

then added 100 nmol · L-1 insulin for 30 min.The amount of glucose consumption was determined by detecting the glucose content in cell culture supernatants with glucose oxidase（GOD） assay.Result: TNF-α markedly decreased both basal and insulin-stimulated glucose uptake.This effect was concentration-dependent

and 20 μg · L-1TNF-α decreased glucose uptake of 79.2% and 81.4%（P<0.05） in the basal and 100 μg · L-1 insulin-stimulated conditions

respectively.Conclusion:20 μg · L-1TNF-α can induce insulin resistance in 3T3-L1 adipocytes.A novel and reliable cell model of insulin resistance is successfully established.

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Related Author

ZHANG Chang-hua

HU Xian-hong

ZHANG Zhong-wei

LIU Yu-hui

YU Ri-yue

XU Guo-liang

WANG Chun-yi

LI Wei-min

Related Institution

Guang'anmen Hospital，China Academy of Chinese Medicine Sciences

First Teaching Hospital of Tianjin University of Traditional Chinese Medicine（TCM）

Shunyi Hospital，Beijing TCM Hospital

Liaoning University of Traditional Chinese Medicine

Gansu University of Chinese Medicine

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