WANG Qun, LIU Wen, SONG Xin-li, et al. Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 45-48.
WANG Qun, LIU Wen, SONG Xin-li, et al. Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 45-48. DOI: 10.13422/j.cnki.syfjx.2013.03.027.
Optimization of Extraction and Purification Technology of Active Components from Evodia rutaecarpa by Multi-indexes
Objective:To optimize extraction and purification technology of Evodia rutaecarpa.Method:With tranfer rate of evodine
evodiamine and rutacarpine as comprehensive evaluation index
influence of extraction times
solvent multiples
solvent concentration and extraction time on alcohol extraction technology of E.rutaecarpa was investigated by orthogonal test;With the mass fraction of evodine
evodiamine and rutacarpine as indexes
single factor test was used to investigate progressive water precipitation purification technology of extraction solution.Result:Optimum extraction technology was as following:refluxing extracted 3 times with 16 times the amount of 70% ethanol
3 hours each time;optimum progressive water precipitation purification technology:recovery of ethanol
centrifugation
precipitate was ultrasonic extracted 15 min with 20 times the amount of 90% ethanol
centrifugation
taking the supernatant
added quarter times of water in second time of water precipitation.Conclusion:Optimized extraction and purification technology could ensure effective components from E.rutaecarpa to maximize retention.
Optimization of Extraction and Purification Technology of Active Components from by Multi-indexes
Study on Liver Injury of Different Components of Evodia Fructus in Vivo and in Vitro
Simultaneous Determination of Evodiamine,Rutaecarpine and Evodin in Cut Crude Drug and Exact of Fructus Evodiae by High-performance Liquid Chromatography
Study on the in vitro Release and Transdermal Permeability of Zuojin Micro-emulsive Gel and Zuojin Aqueous Gel
Comparison the Content of Evodiamine and Rutaecarpine from Compatible Granule, Compound Granule and Traditional Decoction of Zuojin Pills
Related Author
WANG Qun
LIU Wen
SONG Xin-li
SHI Xiao-wei
CHEN Da-ye
YANG Song
Shu-ting WEI
Yun-hua SHENG
Related Institution
China State Institute of Pharmaceutical Industry
Shanghai Institute of Food and Drug Control
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Traditional Chinese Medicine (TCM) Regulating Metabolic Diseases Key Laboratory of Sichuan Province, Hospital of Chengdu University of TCM