TANG Mei1, LIU Jian-yun1, LIU Ying1, et al. Optimization of Impregnating-Squeezing Extraction Technology for Scutellaria baicalensis by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 58-61.
TANG Mei1, LIU Jian-yun1, LIU Ying1, et al. Optimization of Impregnating-Squeezing Extraction Technology for Scutellaria baicalensis by Box-Behnken Design[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 58-61. DOI: 10.13422/j.cnki.syfjx.2013.03.031.
Optimization of Impregnating-Squeezing Extraction Technology for Scutellaria baicalensis by Box-Behnken Design
摘要
目的:优选黄芩的浸渍-压榨提取工艺。方法:采用浸渍-压榨联合提取法
选取提取时间、提取温度、溶剂倍数为考察因素
以总黄酮、黄芩苷提取率和出膏率的总评归一值(OD)为指标
通过Box-Behnken试验设计优选提取工艺。结果:最佳浸渍-压榨提取工艺为加11倍量水于60℃浸泡4 h
压榨
药渣加2倍量水洗涤后压榨
重复操作1次。总黄酮、黄芩苷提取率分别为17.630%
9.38%
出膏率42.7%
与模型预测值非常接近
说明该模型比较可靠。结论:优选的提取工艺科学、合理、可行
为中药常温提取工艺推广提供参考。
Abstract
Objective:To optimize impregnating-squeezing extraction technology for Scutellaria baicalensis.Method:Impregnating-squeezing joint extraction method was used
with OD of extract rate
yield of total flavonoids and baicalin as index
extraction time
extraction temperature and dosage of solvent were selected as factors
extraction technology was optimized by Box-Behnken design.Result:Optimal impregnating-squeezing extraction technology was as follows:soaked 4 h with 11 times the amount of water at 60 ℃
squeezing extracted
add 2 times water to wash dregs
squeezing extracted
once again.Yield of total flavonoids and baicalin were 17.63%
9.38%
respectively
extract rate was 42.7%
these datas were very close to model predictor
it showed this model was reliable.Conclusion:Optimized extraction technology was scientific
reasonable and feasible
it could provide a reference for promoting ordinary temperature extraction technology.
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Related Author
TANG Mei
LIU Jian-yun
LIU Ying
YANG Jin-hui
YANG Ming
CHEN Mengjie
LIU Yimin
ZHOU Yun
Related Institution
Research Center for Differentiation and Development of Traditional Chinese Medicine (TCM) Basic Theory, Jiangxi Province Key Laboratory of TCM Etiopathogenisis, Jiangxi University of Chinese Medicine
Henan Academy of Chinese Medicine
The Second Clinical Medical College, Henan University of Chinese Medicine
State Key Laboratory of Functions and Applications of Medicinal Plants,School of Pharmaceutical Sciences,Engineering Center of Microbiology and Biochemical Pharmacy,Experimental Animal Center,Guizhou Medical University
Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences