Simultaneous Determination of Four Components in Sijunzi Tang by HPLC

XU Qiu-xiang1; YU Hong-li1; WU Hao1; GONG Le1; PAN Yao-zong1; QIU Yun-ying1

doi:10.13422/j.cnki.syfjx.2013.03.032

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Simultaneous Determination of Four Components in Sijunzi Tang by HPLC

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- Simultaneous Determination of Four Components in Sijunzi Tang by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 62-65(2013)
- 作者机构：
  
  1. 南京中医药大学药学院
  2. 2. 江苏省中药炮制重点实验室 3. 国家教育部中药炮制规范及标准工艺研究中心
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.032
  CLC： R284
- Published：2013
- 稿件说明：
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[1]徐秋香,郁红礼,吴皓,宫乐,潘耀宗,邱韵萦.HPLC同时测定四君子汤中4种指标性成分的含量[J].中国实验方剂学杂志,2013,19(03):62-65.

XU Qiu-xiang1, YU Hong-li1, WU Hao1, et al. Simultaneous Determination of Four Components in Sijunzi Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 62-65.
[1]徐秋香,郁红礼,吴皓,宫乐,潘耀宗,邱韵萦.HPLC同时测定四君子汤中4种指标性成分的含量[J].中国实验方剂学杂志,2013,19(03):62-65. DOI： 10.13422/j.cnki.syfjx.2013.03.032.

XU Qiu-xiang1, YU Hong-li1, WU Hao1, et al. Simultaneous Determination of Four Components in Sijunzi Tang by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 62-65. DOI： 10.13422/j.cnki.syfjx.2013.03.032.

摘要

目的:建立同时测定四君子汤中人参皂苷Rg1、人参皂苷Re、人参皂苷Rb1和甘草酸含量的方法。方法:采用HPLC测定

Thermo Hypersil BDS色谱柱（4.6 mm×250 mm

5μm）

流动相为乙腈-0.03%甲酸梯度洗脱

流速0.9 mL.min-1

柱温30℃

检测波长202

250 nm。结果:人参皂苷Rg1在1.522～12.176μg线性关系良好

平均回收率为100.86%

RSD2.34%

人参皂苷Re在1.368～10.944μg线性关系良好

平均回收率为100.57%

RSD 2.95%;人参皂苷Rb1在3.198～15.990μg线性关系良好

平均回收率97.88%

RSD 2.86%;甘草酸在5.036～40.286μg线性关系良好

平均回收率99.25%

RSD 2.97%。结论:该方法简便、准确、实用性强

可用于四君子汤中4种指标性成分的含量测定。

Abstract

Objective:To establish the method for determining simultaneously four components in Sijunzi Tang

ginsenosid Rg1

ginsenosid Re

ginsenosid Rb1 and glycyrrhizic acid.Method:HPLC was carried out on the Hypersil BDS column（4.6 mm× 250 mm

5 μm） with acetonitrile-0.03% phosphoric acid

gradient elution

the volume of flow 0.9 mL · min-1

column temperature 30 ℃

detection wavelength 202 nm and 250 nm.Result:The correlation coefficients of the four components were higher than 0.999 and the average recoveries were 100.86%

100.57%

97.88%

99.25%

respectively;and RSD of the above four components were 2.34%

2.95%

2.86%

2.97%

respectively.Conclusion:The method is proved to be so easy and accurate and practical that it can be used to determine the four components in Sijunzi Tang.

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Related Author

XU Qiu-xiang

YU Hong-li

WU Hao

GONG Le

PAN Yao-zong

QIU Yun-ying

LI Hai-yan1

JIN Xiang-qun1

Related Institution

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School of Life Sciences, Beijing University of Chinese Medicine

School of Pharmacy, Ningxia Medical University

Jiangxi University of Traditional Chinese Medicine

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