Investigate the Best Time of Decoction of Gastrodiae Rhizoma

GAO Xiao-ling; LI Guang-long; WANG Shu-ling; ZENG Yuan-er

doi:10.13422/j.cnki.syfjx.2013.03.036

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Investigate the Best Time of Decoction of Gastrodiae Rhizoma

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- Investigate the Best Time of Decoction of Gastrodiae Rhizoma
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 83-85(2013)
- 作者机构：
  
  广州中医药大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.036
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]高晓玲,李光龙,王术玲,曾元儿.天麻最佳煎煮时间的探讨[J].中国实验方剂学杂志,2013,19(03):83-85.

GAO Xiao-ling, LI Guang-long, WANG Shu-ling, et al. Investigate the Best Time of Decoction of Gastrodiae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 83-85.
[1]高晓玲,李光龙,王术玲,曾元儿.天麻最佳煎煮时间的探讨[J].中国实验方剂学杂志,2013,19(03):83-85. DOI： 10.13422/j.cnki.syfjx.2013.03.036.

GAO Xiao-ling, LI Guang-long, WANG Shu-ling, et al. Investigate the Best Time of Decoction of Gastrodiae Rhizoma[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 83-85. DOI： 10.13422/j.cnki.syfjx.2013.03.036.

摘要

目的:探讨天麻水溶性成分的煎出量与煎煮时间的关系。方法:采用高效液相色谱法

phenomenex C18（4.6 mm×250 mm

5μm）色谱柱

流动相乙腈与0.2%磷酸

梯度洗脱

流速1 mL.min-1

检测波长220 nm。结果:天麻素在0.354 4～3.544μg线性关系良好（r=0.999）

平均回收率为99.4%

RSD 1.2%

天麻在煎煮3 h时天麻素等6个主要成分的煎出量最大。结论:天麻药材的最佳煎煮时间为3 h

与传统要求的久煎相吻合。

Abstract

Objective:To investigate the relationship between the amount of water-soluble components of gastrodia and the boiling time.Method: A phenomenex C18（4.6 mm×250 mm

5 μm） column was adopted.The mobile phase consisted of acetonitrile（A） and 0.2% phosphoric acid solution（B） in gradient elution mode.The flow rate was 1.0 mL · min-1.The detection wavelength was set at 220 nm.Result: The linear range of gastrodin was 0.354 4-3.544 μg（r>0.999）.The average recovery was 99.4%

RSD 1.2%.The maximum amounts of six major components including gastrodin were decocted 3 hours.Conclusion: The best time of decoction of Gastrodiae Rhizoma was 3 hours

consistent with traditional requirements.

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Related Author

GAO Xiao-ling

LI Guang-long

WANG Shu-ling

ZENG Yuan-er

Fan LIU

Ting-ting CHEN

Yan CHEN

Zi-peng GONG

Related Institution

Provincial Key Laboratory of Pharmaceutics in Guizhou Province, State Key Laboratory of Functions and Applications of Medicinal Plants, Engineering Research Center for Development and Application of Ethnic Medicine and Traditional Chinese Medicine, Ministry of Education, School of Pharmaceutical Sciences, Guizhou Medical University

The Affiliated Hospital of Guizhou Medical University

Anhui Province Key Laboratory of Traditional Chinese Medicine，School of Pharmacy， Anhui University of Chinese Medicine

State Key Laboratory of Efficacy and Utilization of Medicinal Plants，Guizhou Medical University

Henan University of Chinese Medicine

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