Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC

KONG Ming1; ZHANG Jian1; YAO Nan1; LI Yue1; JIANG Cui-hua1; GAO Meng1; FANG Zhi-jun1; LIANG Jing-yu2

doi:10.13422/j.cnki.syfjx.2013.03.039

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Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC

更新时间：2024-09-23

- Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 93-96(2013)
- 作者机构：
  
  1. 江苏省中医药研究院
  2. 中国药科大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.039
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]孔铭,张健,姚楠,李玥,蒋翠花,高萌,方志军,梁敬钰.HPLC同时测定络石藤中5个化学成分的含量[J].中国实验方剂学杂志,2013,19(03):93-96.

KONG Ming1, ZHANG Jian1, YAO Nan1, et al. Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 93-96.
[1]孔铭,张健,姚楠,李玥,蒋翠花,高萌,方志军,梁敬钰.HPLC同时测定络石藤中5个化学成分的含量[J].中国实验方剂学杂志,2013,19(03):93-96. DOI： 10.13422/j.cnki.syfjx.2013.03.039.

KONG Ming1, ZHANG Jian1, YAO Nan1, et al. Simultaneous Determination of Five Compounds in Trachelospermum jasminodes by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 93-96. DOI： 10.13422/j.cnki.syfjx.2013.03.039.

摘要

目的:建立测定中药络石藤中络石苷元-4’-O-β-龙胆二糖苷、络石苷、野漆树苷、络石苷元及木犀草素含量的HPLC方法。方法:75%甲醇提取络石藤药材

微孔滤膜（0.45μm）过滤

HPLC测定

采用Alltima C18色谱柱（4.6 mm×250mm

5μm）

以0.5%HAc-甲醇为流动相梯度洗脱

流速1.0 mL.min-1

检测波长270 nm

采用外标法峰面积计算含量。结果:5种成分均达到基线分离

线性关系良好

精密度RSD<2.0%。该法成功的用于络石藤药材中5种成分含量的测定。药材中络石苷元-4’-O-β-龙胆二糖苷、络石苷、野漆树苷、络石苷元及木犀草素的平均回收率分别为98.27%

97.30%

97.20%

96.89%

96.48%。结论:方法操作简单、结果准确

具有较好的重复性和稳定性

为络石藤药材质量控制提供了依据。

Abstract

Objective:To establish a method for determination of trachelogenin-4′-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin in Trachelospermum jasminoides.Method: The Alltima C18 column（4.6 mm×250 mm

5 μm） was used;the mobile phase consisting of 0.5%HAc-water.The flow rate was 1.0 mL · min-1;the detection wavelength set at 270 nm and content calculated with external reference method. Result: The five compounds were well separated.Calibration curves showed good linear relationship

RSD<2.0%.The average recoveries of trachelogenin-4′-O-β-gentiobioside

tracheloside

rhoifolin

trachelogenin and luteolin were 98.27%

97.30%

97.20%

96.89% and 96.48% respectively.Conclusion: The developed method is simple

accurate

and repeatable

which can be readily used as valid tool for the quality control of T.Jasminoides.

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Related Author

KONG Ming

ZHANG Jian

YAO Nan

LI Yue

JIANG Cui-hua

GAO Meng

FANG Zhi-jun

LIANG Jing-yu

Related Institution

Shandong Academy of Traditional Chinese Medicine

Taian Institute for Drug Control,Tai′an

Jilin Agricultural University

Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences

Chengde Medical University

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