LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Baicalin in Qinxuan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 110-112.
LI Xiang, LIU Gui-yang, MA Jian-li, et al. Determination of Baicalin in Qinxuan Granules by HPLC[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 110-112. DOI: 10.13422/j.cnki.syfjx.2013.03.044.
Determination of Baicalin in Qinxuan Granules by HPLC
Objective:To establish an HPLC method for determination of baicalin in Qinxuan granules.Method: The Agilent Eclipse XDB-C18 was used.The mobile phase consisted of methanol-0.5% acetic acid(45∶55)
the flow rate was 1.0 mL · min-1
the detection wavelength was at 277 nm
the column temperature was kept at 30 ℃
the inject volume was 10 μL.Result: A good linear correlation of baicalin was observed within the range of 2.128-106.4 μg·min-1.The average recovery was 101.50% with RSD 1.00%(n=6).Conclusion: The method is rapid and accurate;it can be used for the determination of baicalin in Qinxuan granules.