Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis

MA Wen-fang1; DENG Hui-lian1; CAI Yi1; YU Jiao1

doi:10.13422/j.cnki.syfjx.2013.03.045

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Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis

更新时间：2024-09-23

- Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 112-115(2013)
- 作者机构：
  
  1. 广西中医药大学
  2. 成都中医药大学
  3. 浙江省安吉县妇幼保健院药剂科
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.045
  CLC： R284.1
- Published：2013
- 稿件说明：
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[1]马雯芳,邓慧连,蔡毅,余娇.冰糖草总黄酮含量测定及显色方法优化[J].中国实验方剂学杂志,2013,19(03):112-115.

MA Wen-fang1, DENG Hui-lian1, CAI Yi1, et al. Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 112-115.
[1]马雯芳,邓慧连,蔡毅,余娇.冰糖草总黄酮含量测定及显色方法优化[J].中国实验方剂学杂志,2013,19(03):112-115. DOI： 10.13422/j.cnki.syfjx.2013.03.045.

MA Wen-fang1, DENG Hui-lian1, CAI Yi1, et al. Research on the Determination of Total Flavonoids Content and Colour Reaction System in Scoparia dulcis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 112-115. DOI： 10.13422/j.cnki.syfjx.2013.03.045.

摘要

目的:建立用紫外-可见分光光度法测定冰糖草总黄酮含量的方法。方法:对显色剂的种类、浓度与用量进行考察

采用NaNO2-Al（NO3）3-NaOH显色反应体系

测定冰糖草中总黄酮含量。结果:以芦丁为对照品

测定波长为506 nm

在4.8～96.2 mg.L-1线性关系良好（r=0.999 3）

平均回收率为103.71%（RSD 2.68%

n=6）。结论:该方法简便、快捷、准确

可作为冰糖草总黄酮含量测定的方法。

Abstract

Objective:To establish a quantitative method for the determination of total flavonoids in Scoparia dulcis by UV-VIS spectrophotometry.Method: The effects of species

concentration

and dosage of chromogenic reagent were studied

and NaNO2-Al（NO3）3-NaOH colour reaction system was used for content determination of total flavonoids in Scoparia dulcis.Result: Using rutin as comparison sample

the detection wave length was set at 506 nm.There was good linear relationship during 4.8-96.2 mg · L-1（r=0.999 3）.The average recovery rate was 103.71%（RSD 2.68%

n=6）.Conclusion: The method is simple

rapid

accurate and can be used for the quantitative method of S.dulcis.

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Related Author

MA Wen-Fang

DENG Hui-lian

CAI Yi

YU Jiao

CHEN Shi-qiang

LIN Jiang-xiao

CHEN Jian-feng

LIU Guo-min

Related Institution

Pharmacy Technology Center，Tsinghua University

Center of Pharmacy R&D，XinJiang Huachun Bio-Pharmaceutical Co. Ltd.

School of Life Sciences， Tsinghua University

Institute of Medicinal Plant Development，Chinese Academy of Medical Sciences and Peking Union Medical College

School of Pharmacy， Henan University of Chinese Medicine

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