Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens

ZHANG Yan-zhen; DONG Cheng-ming; SU Xiu-hong; CHAO Li-hua

doi:10.13422/j.cnki.syfjx.2013.03.052

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Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens

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- Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 132-135(2013)
- 作者机构：
  
  河南中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.052
  CLC： R284
- Published：2013
- 稿件说明：
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[1]张艳贞,董诚明,苏秀红,曹利华.稀土元素对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响[J].中国实验方剂学杂志,2013,19(03):132-135.

ZHANG Yan-zhen, DONG Cheng-ming, SU Xiu-hong, et al. Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 132-135.
[1]张艳贞,董诚明,苏秀红,曹利华.稀土元素对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响[J].中国实验方剂学杂志,2013,19(03):132-135. DOI： 10.13422/j.cnki.syfjx.2013.03.052.

ZHANG Yan-zhen, DONG Cheng-ming, SU Xiu-hong, et al. Effect of Rare-earth Elements on Callus Growth and Oridonin and Ponicidin in Rabdosia rubescens[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 132-135. DOI： 10.13422/j.cnki.syfjx.2013.03.052.

摘要

目的:探讨稀土元素镧、铈对冬凌草愈伤组织生长及冬凌草甲素、乙素含量的影响。方法:冬凌草均匀愈伤组织在添加不同浓度La3+或Ce3+的培养基中培养

通过质量法测定愈伤组织鲜质量、绘制生长曲线

HPLC测定甲素、乙素含量。结果:CeCl3.7H2O浓度为1μmol.L-1时

愈伤生物量最高

是空白条件下的2.4倍。空白及对照条件下甲素含量均为0;空白条件下乙素含量最低为0.11%

随着Ce3+浓度升高而升高

当浓度为10μmol.L-1时高达0.58%;乙素随着La3+浓度升高而降低

当浓度为0.1μmol.L-1时含量高达0.44%。结论:0～10μmol.L-1的La3+及Ce3+均有利于冬凌草愈伤生物量的积累及乙素的合成

对甲素的合成无影响

将CeCl3.7H2O浓度10μmol.L-1作为最佳条件选择。

Abstract

Objective:To study the effect of rare-earth elements La3+and Ce3+ on callus growth and oridonin and ponicidin in Rabdosia rubescens.Method: The uniform callus of R.rubescens was cultivated in different concentration of La3+or Ce3+ in culture medium

and the gravimetric method was used for the determination of callus fresh weight and cell growth curve;HPLC method was used for the determination of oridonin and ponicidin content.Result: The supreme biomass of callus was 2.4 times of blank conditions when CeCl3 ·7H2O concentration was 1 μmol · L-1.Blank and the control conditions of oridonin content were 0;ponicidin was the lowest with 0.11% at blank condition;the ponicidin concentration increased as Ce3+ increasing

it was up to 0.58% when Ce3+ concentration was 10 μmol · L-1 while the ponicidin concentration decreased as La3+increasing

the content was 0.44% when La3+ concentration was 0.1 μmol · L-1.Conclusion: 0-10 μmol · L-1 La3+ and Ce3+ promoted R.rubescens callus biomass accumulation and ponicidin synthesis

didn′t promote the synthesis of oridonin

the CeCl3 · 7H2O concentration of 10 μmol · L-1 was the selection of optimum conditions.

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