Effects of the Compounds Isolated from Urtica laetevirens on Cell Proliferation and Apoptosis in MH7A Cells

PENG Qian; YU Da-yong; SHI Li-ying; FENG Bao-min; TANG Ling; WANG Yong-qi

doi:10.13422/j.cnki.syfjx.2013.03.061

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Effects of the Compounds Isolated from Urtica laetevirens on Cell Proliferation and Apoptosis in MH7A Cells

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- Effects of the Compounds Isolated from Urtica laetevirens on Cell Proliferation and Apoptosis in MH7A Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 172-174(2013)
- 作者机构：
  
  大连大学药物研究所
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.061
  CLC： R285
- Published：2013
- 稿件说明：
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[1]彭倩,于大永,史丽颖,冯宝民,唐玲,王永奇.宽叶荨麻中化学成分对MH7A细胞增殖和凋亡的影响[J].中国实验方剂学杂志,2013,19(03):172-174.

PENG Qian, YU Da-yong, SHI Li-ying, et al. Effects of the Compounds Isolated from Urtica laetevirens on Cell Proliferation and Apoptosis in MH7A Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 172-174.
[1]彭倩,于大永,史丽颖,冯宝民,唐玲,王永奇.宽叶荨麻中化学成分对MH7A细胞增殖和凋亡的影响[J].中国实验方剂学杂志,2013,19(03):172-174. DOI： 10.13422/j.cnki.syfjx.2013.03.061.

PENG Qian, YU Da-yong, SHI Li-ying, et al. Effects of the Compounds Isolated from Urtica laetevirens on Cell Proliferation and Apoptosis in MH7A Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 172-174. DOI： 10.13422/j.cnki.syfjx.2013.03.061.

摘要

目的:探讨从具有抗类风湿关节炎作用的宽叶荨麻中分离到的9个化学成分对人类风湿性关节炎滑膜成纤维细胞MH7A增殖的影响。方法:分别以从宽叶荨麻中分离得到的9个化学成分（0～400 mg.L-1）:RoseosideⅡ（C1）

（6S

9R）-3-氧-α-紫罗兰醇-β-D-葡萄糖苷（C2）

（6R

9R）-3-氧-α-紫罗兰醇-β-D-葡萄糖基（1→2）-β-D-葡萄糖苷（C3）

芹菜素-6

8-二-C-β-D-吡喃葡萄糖苷（C4）

木樨草素-7-O-新橙皮糖苷（C5）

木樨草素-7-O-β-D-吡喃葡萄糖苷（C6）

芦丁（C7）

异落叶松脂素-9-O-β-D-吡喃葡萄糖苷（C8）

5α

6β-二羟基-胡萝卜苷（C9）处理MH7A细胞（2.5×104/mL）

24 h后采用MTT法检测细胞增殖率

并通过测定DNA片段化检测细胞凋亡率。结果:在被检测的9个化合物中

化合物5α

6β-二羟基-胡萝卜苷显示了较强的抑制MH7A细胞增殖的作用

其IC50值为140 mg.L-1。DNA片段化分析结果表明

其能显著诱导MH7A细胞凋亡。结论:宽叶荨麻中化学成分5α

6β-二羟基-胡萝卜苷能够显著抑制MH7A细胞增殖并能诱导MH7A细胞凋亡。

Abstract

Objective:The antiproliferative and apoptosis inducing effects of the compounds isolated from Urtica laetevirens on MH7A human rheumatoid arthritis synovial cells were tested in this study.Method: MH7A cells were treated with nine compounds isolated from U.laetevirens

respectively.The antiproliferative activities of nine compounds were evaluated by MTT assay

and the apoptosis was assessed with DNA fragmentation after 24 h.Result: Among the nine compounds 5α

6β-dihydroxy-daucosterol showed stronger antiproliferative activity against MH7A cells.It could induce DNA fragmentation in MH7A cells.Conclusion: The compound

5α

6β-dihydroxy-daucosterol isolated from U.laetevirens could inhibit cell proliferation and induce apoptosis in MH7A cell.

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Related Author

PENG Qian

YU Da-yong

SHI Li-ying

FENG Bao-min

TANG Ling

WANG Yong-qi

LIN Na

ZHANG Yanqiong

Related Institution

Institute of Chinese Materia Medica，China Academy of Chinese Medical Sciences

Southern Medical University Hospital of Integrated Chinese and Western Medicine

Tongji Hospital， Tongji Medical College of Huazhong University of Science and Technology

School of Chinese Materia Medica， Beijing University of Chinese Medicine

School of Pharmacy，Chengdu University of Traditional Chinese Medicine

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