Antioxidant Activities in vitro of Polysaccharides from Acalypha australis

WEI Xiu-juan; XIANG Fa-chun; CUI Ming-jun; XU Jian-rong

doi:10.13422/j.cnki.syfjx.2013.03.066

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Antioxidant Activities in vitro of Polysaccharides from Acalypha australis

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- Antioxidant Activities in vitro of Polysaccharides from Acalypha australis
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 197-200(2013)
- 作者机构：
  
  西南科技大学生命科学与工程学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.066
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]魏秀娟,向发椿,崔明筠,徐健蓉.铁苋菜多糖体外抗氧化研究[J].中国实验方剂学杂志,2013,19(03):197-200.

WEI Xiu-juan, XIANG Fa-chun, CUI Ming-jun, et al. Antioxidant Activities in vitro of Polysaccharides from Acalypha australis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 197-200.
[1]魏秀娟,向发椿,崔明筠,徐健蓉.铁苋菜多糖体外抗氧化研究[J].中国实验方剂学杂志,2013,19(03):197-200. DOI： 10.13422/j.cnki.syfjx.2013.03.066.

WEI Xiu-juan, XIANG Fa-chun, CUI Ming-jun, et al. Antioxidant Activities in vitro of Polysaccharides from Acalypha australis[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 197-200. DOI： 10.13422/j.cnki.syfjx.2013.03.066.

摘要

目的:研究不同醇沉浓度下铁苋菜多糖的体外抗氧化活性。方法:用质量浓度为30%

45%

60%

75%

80%的无水乙醇分级沉淀的方法得到不同组分的铁苋菜多糖

分别采用高铁还原法、对二苯代苦味酰基（DPPH.）体系、光照核黄素法、硫代巴比妥酸法比较它们的总还原力（T-AOC）、对二苯代苦味酰基自由基（DPPH.）、超氧阴离子自由基（O2-.）、羟基自由基（.OH）的清除作用及对卵黄脂蛋白脂质过氧化物（LPO）的抑制效果。结果:醇沉浓度为75%时对DPPH.的清除率达到了73.85%

其余各组分多糖都有良好的抗氧化效果

其中对O2-.和.OH的清除作用最为明显

清除率分别达到了92.47%和95.89%

几乎与抗坏血酸的清除作用相当

75%醇沉条件下的多糖对LPO也有较强的抑制作用。结论:铁苋菜各组分多糖都有较强的抗氧化作用。

Abstract

Objective:To assess the antioxidant activities of polysaccharide from Acalypha australis L.by different ethanol concentration in vitro.Method:The different part of polysaccharide were precipitated by 30%

45%

60%

75%

80% ethanol.The scavenging rate of the precipitated polysaccharides on（DPPH ·）free radical

superoxide anion radical（O-2 ·）

hydroxyl radicals（· OH） was investigated by ferric reducing method

DPPH · radical assay

O-2 · radical assay and thiobarbituric acid method respectively.The total antioxidantive capacity（T-AOC） and the inhibition on lipid peroxide（LPO） of the precipitated polysaccharides were also been determined.Result:The scavenging activities on the DPPH · reached 73.85%.The results showed that T-AOC was enhanced with the increase of polysaccharide concentration.The antioxidantive especially the scavenging activities on the O-2 · and · OH reached 92.47%

95.89% respectively at 5 g · L-1.Furthermore the scavenging effects were as much as that of vitamin C（VC）.The inhibition of LPO with the polysaccharide precipitated by 75% ethanol was significantly higher than that of any by other ethanol concentrations.Conclusion:the precipitated polysaccharides from A.australis showed strong antioxidant activities.

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