Effect of Liuwei Dihuang Wan on the Learning Memory and Cholinergic M1 Receptor Positive Neurons of Aged Rats

CHEN Qiao; LI Zheng-feng; LI Qin; HOU Ji-hua; WU Jun-xia; WANG Jian-min

doi:10.13422/j.cnki.syfjx.2013.03.068

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Effect of Liuwei Dihuang Wan on the Learning Memory and Cholinergic M1 Receptor Positive Neurons of Aged Rats

更新时间：2024-09-23

- Effect of Liuwei Dihuang Wan on the Learning Memory and Cholinergic M1 Receptor Positive Neurons of Aged Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 205-208(2013)
- 作者机构：
  
  江西中医学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.068
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]陈乔,李征峰,李青,侯吉华,吴俊霞,汪建民.六味地黄丸对老年大鼠学习记忆及脑内M1胆碱受体阳性神经元的影响[J].中国实验方剂学杂志,2013,19(03):205-208.

CHEN Qiao, LI Zheng-feng, LI Qin, et al. Effect of Liuwei Dihuang Wan on the Learning Memory and Cholinergic M1 Receptor Positive Neurons of Aged Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 205-208.
[1]陈乔,李征峰,李青,侯吉华,吴俊霞,汪建民.六味地黄丸对老年大鼠学习记忆及脑内M1胆碱受体阳性神经元的影响[J].中国实验方剂学杂志,2013,19(03):205-208. DOI： 10.13422/j.cnki.syfjx.2013.03.068.

CHEN Qiao, LI Zheng-feng, LI Qin, et al. Effect of Liuwei Dihuang Wan on the Learning Memory and Cholinergic M1 Receptor Positive Neurons of Aged Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 205-208. DOI： 10.13422/j.cnki.syfjx.2013.03.068.

摘要

目的:观察六味地黄丸对老年大鼠学习记忆及脑内M1胆碱受体（MI-AchR）阳性神经元的影响

探讨滋阴补肾法改善老年大鼠学习记忆能力的可能机制。方法:将雄性SD大鼠分为青年对照组（3月龄）、老年对照组（24月龄）和六味地黄丸组（24月龄）。六味地黄丸组大鼠按3 g.kg-1ig六味地黄丸液

老年对照组ig同体积生理盐水

各30 d;青年对照组ig同体积生理盐水7 d。通过Morris水迷宫实验

比较其学习记忆能力

并运用免疫组织化学方法

检测大脑海马和皮质相关区域M1-AchR阳性神经元变化。结果:老年对照组各指标与青年对照组比

有极显著性差异（P<0.01）。六味地黄丸组平均逃避潜伏期自第3天起较老年对照组明显缩短

有极显著性差异（P<0.01）。目标象限游程占全部游程百分比方面

与老年对照组相比

六味地黄丸组有极显著性差异（P<0.01）;六味地黄丸组与老年对照组穿越平台次数相比

有极显著性差异（P<0.01）。与老年对照组相比

六味地黄丸组M1-AchR阳性细胞数目增多

在海马CA3区及第一躯体感觉区（S1Tr）内

有极显著性差异（P<0.01）。结论:六味地黄丸可以改善自然衰老大鼠的空间学习记忆能力

可能与其保护海马CA3区及S1Tr内胆碱能毒蕈碱M1-AchR阳性神经元

增强中枢胆碱能系统功能有关。

Abstract

Objective:To study the effect of Liuwei Dihuang Wan on aged rats with the learning memory and cholinergic M1 receptor（M1-AchR） positive neurons.Method: The SD rats were divided into three groups: healthy young control group﹙3 month-old﹚

the natural aging model group ﹙24 month-old﹚and the Liuwei Dihuang Wan group﹙24 month-old﹚.Liuwei Dihuang Wan group and the natural aging model group were orally administered with Liuwei Dihuang Wan﹙3 g·kg-1﹚and the same volume of distilled water for thirty days;the healthy young control group was administered with the same volume of distilled water for seven days.It tested the learning and memory capacity with Morris water maze.It detected M1-AchR with immunohistochemistry.Result:All of the indexes between rats in older group and the healthy young control group were significant（P<0.01）.The average escape latency of the elderly was significantly shorter than the Liuwei Dihuang Wan group since the third day

and there was a significant difference（P<0.01）.Space exploration experiments showed that there was a significant difference between rats in older group and Liuwei Dihuang Wan group in the number of crossing the platform（P<0.01）.Compared with the aged rats in control group

the numbers of M1-AchR positive cell of aged rats in Liuwei Dihuang Wan group increased in the hippocampus CA3 and primary somatosensory cortex;trunk region（S1Tr）

and there were very significant between them（P<0.01）.Conclusion: Liuwei Dihuang Wan can improve the learning and memory of the natural aging rats

which was caused possibly by protecting M1 AchR positive neurons in the hippocampal CA3 and S1Tr and enhanced the central cholinergic system function.

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Related Author

CHEN Qiao

LI Zheng-feng

LI Qin

HOU Ji-hua

WU Jun-xia

WANG Jian-min

LIU Huizhen

ZHANG Yini

Related Institution

School of Basic Medical Sciences， Hubei University of Chinese Medicine

Hubei Shizhen Laboratory

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Engineering Research Center of Traditional Chinese Medicine Protection Technology and New Product Development for the Elderly Brain Health， Ministry of Education， Hubei University of Chinese Medicine

School of Traditional Chinese Medicine， Hubei University of Chinese Medicine

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