Effect of Puerarin on the Expressions of BDNF,TrkB,caspase-3 in Substantia Nigra Tissue of Parkinson’s Rats

LI Rong1; XU Ling-yuan2; LIANG Tao3; ZHANG Shi-jun3; LI Yong-wen1; DUAN Xiao-qun1

doi:10.13422/j.cnki.syfjx.2013.03.069

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Effect of Puerarin on the Expressions of BDNF,TrkB,caspase-3 in Substantia Nigra Tissue of Parkinson’s Rats

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- Effect of Puerarin on the Expressions of BDNF,TrkB,caspase-3 in Substantia Nigra Tissue of Parkinson’s Rats
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 208-211(2013)
- 作者机构：
  
  1. 桂林医学院
  2. 右江民族医学院附属医院药剂科
  3. 广西医科大学
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.069
  CLC： R285.5
- Published：2013
- 稿件说明：
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[1]黎荣,徐灵源,梁韬,张士军,李勇文,段小群.葛根素对帕金森病大鼠黑质组织BDNF,TrkB,caspase-3表达的影响[J].中国实验方剂学杂志,2013,19(03):208-211.

LI Rong1, XU Ling-yuan2, LIANG Tao3, et al. Effect of Puerarin on the Expressions of BDNF,TrkB,caspase-3 in Substantia Nigra Tissue of Parkinson’s Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 208-211.
[1]黎荣,徐灵源,梁韬,张士军,李勇文,段小群.葛根素对帕金森病大鼠黑质组织BDNF,TrkB,caspase-3表达的影响[J].中国实验方剂学杂志,2013,19(03):208-211. DOI： 10.13422/j.cnki.syfjx.2013.03.069.

LI Rong1, XU Ling-yuan2, LIANG Tao3, et al. Effect of Puerarin on the Expressions of BDNF,TrkB,caspase-3 in Substantia Nigra Tissue of Parkinson’s Rats[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 208-211. DOI： 10.13422/j.cnki.syfjx.2013.03.069.

摘要

目的:研究葛根素对6-羟多巴胺（6-OHDA）致帕金森病大鼠黑质组织脑原性神经营养因子（BDNF）

酪氨酸激酶B（TrkB）

半胱氨酸天冬酶-3（caspase-3）表达的影响。方法:建立帕金森病大鼠模型

随机分成5组:模型组、左旋多巴阳性组及葛根素低、中、高剂量组。ig给予葛根素20

80 mg.kg-1

阳性组给予左旋多巴40 mg.kg-1

连续灌胃30 d。TUNEL检测法观察黑质神经细胞的凋亡情况

Western blot法检测黑质组织BDNF

TrkB表达。免疫组织化学法检测caspase-3表达。结果:与模型组比较

葛根素（20

80 mg.kg-1）有效地降低黑质组织神经细胞凋亡水平（11.94±2.57）

（7.61±1.21）

（4.35±0.36）cell.mm-2（P<0.01）。上调黑质组织中BDNF、TrkB蛋白表达20.45%

31.29%

45.36%;14.76%

27.65%

38.81%（P<0.01）。而caspase-3免疫阳性细胞数量明显减少（36.48±6.25）

（22.74±4.03）

（12.19±2.51）cell.mm-2（P<0.01）。结论:葛根素对6-OHDA所致PD大鼠黑质神经细胞具有保护作用

机制可能与通过神经修复或再生以及逆转细胞凋亡进程而改善黑质功能有关。

Abstract

Objective:To investigate the effect of puerarin on the expressions of brain-derived neurotrophic factor（BDNF）

tyrosin kinase B（TrkB）

caspase-3 in substantia nigra tissue of Parkinson’s rats induced by 6-hydroxydopamine（6-OHDA）.Method: Rat parkinson model was established

themodeling rats were randomly divided into 5 groups: model group

L-dopa group（40 mg · kg-1）

low-

medium-and high-dosage groups of puerarin（20

80 mg · kg-1）.The drugs were intragastrically perfused daily for 30 consecutive days.The apoptosis of nerve cells in substantia nigra was observed by TUNEL assay.The expression of BDNF and TrkB in substantia nigra was assayed by Western blot analysis.The caspase-3 expression was tested by immunohistochemistry assay.Result: Compared with model control group

puerarin effectively reduced neuronal apoptosis levels in substantia nigra tissue of parkinson’s rats（P<0.01）

and up-regulated the BDNF

TrkB protein levels in substantia nigra tissue（P<0.01）.The number of caspase-3 immunoreactive cells was significantly decreased.Conclusion: The results demonstrate that puerarin has protective effect on the neuronal cells in substantia nigra of PD rats induced by 6-OHDA

probably the mechanism may be linked to neuronic repair or regeneration and reverse the process of apoptosis to improve the functions in substantia nigra.

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Related Author

LI Rong

XU Ling-yuan

LIANG Tao

ZHANG Shi-jun

LI Yong-wen

DUAN Xiao-qun

WEI Bin

XUN Ling-yuan

Related Institution

Affiliated Hospital of Youjiang Medical University for Nationalities

The First Hospital of Hunan University of Chinese Medicine

People's Hospital of Ningxiang City，Ningxiang

Gansu University of Chinese Medicine

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