ZHANG Zhi-bin, LU Shu, ZHOU Chun-gang, et al. Intervention of Danggui Buxue Decoction on Apoptosis in Rat Arterial Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 215-218.
ZHANG Zhi-bin, LU Shu, ZHOU Chun-gang, et al. Intervention of Danggui Buxue Decoction on Apoptosis in Rat Arterial Endothelial Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 215-218. DOI: 10.13422/j.cnki.syfjx.2013.03.071.
Intervention of Danggui Buxue Decoction on Apoptosis in Rat Arterial Endothelial Cells
摘要
目的:研究当归补血汤对动脉血管内皮细胞凋亡的影响
探讨其可能作用机制。方法:当归补血汤提取液作用于血管紧张素Ⅱ(AngⅡ)诱导的大鼠动脉内皮细胞(RAOEC)凋亡模型(细胞密度1×105/mL)24 h
Objective:To study effect of Danggui Buxue decoction on apoptosis of rat arterial endothelial cells(RAOECs)and its mechanism.Method: Extracts of Danggui Buxue decoction was incubated with angiotensinⅡ-induced apoptotic RAOECs(1×105/mL) for 24 hours
apoptosis rate and mRNA level of caspase-8
B cell lymphoma/leukmia-2 Bcl-2 and Bcl-2 assaciated x protein(Bax) were detected.Result: Apoptosis rate in 500 mg · L-1 Danggui Buxue decoction group was(8.69±0.69)%
which significantly lower than that in control group(14.45±1.87)%(P<0.05);while mRNA level of caspase-8
Bax and Bcl-2 of Danggui Buxue decoction group(0.603±0.081
0.629±0.079
0.566±0.113
respectively)were all significantly lower than that in control group(1.083±0.115
1.024±0.072
1.018±0.111
respectively)
(all P<0.05).Conclusion: Danggui Buxue decoction has effects on apoptosis of RAOECs.Danggui Buxue decoction may inhibit RAOECs apoptosis by down-regulating caspase-8 mRNA expression and mitochondrial signaling pathway by down-regulating Bax mRNA expression respectively.