Study of Mailuoning Cause an Allergic the RBL-2H3 Cells

YU Hai-bin1; LIU Qing2; CUI Lin1

doi:10.13422/j.cnki.syfjx.2013.03.079

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Study of Mailuoning Cause an Allergic the RBL-2H3 Cells

更新时间：2024-09-23

- Study of Mailuoning Cause an Allergic the RBL-2H3 Cells
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 3, Pages: 245-248(2013)
- 作者机构：
  
  1. 河南中医学院第一附属医院国家中药管理局中药药理三级实验室
  2. 河南中医学院研究生部
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.03.079
  CLC： R286
- Published：2013
- 稿件说明：
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[1]余海滨,刘青,崔琳.脉络宁针剂致敏RBL-2H3细胞的实验研究[J].中国实验方剂学杂志,2013,19(03):245-248.

YU Hai-bin1, LIU Qing2, CUI Lin1. Study of Mailuoning Cause an Allergic the RBL-2H3 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 245-248.
[1]余海滨,刘青,崔琳.脉络宁针剂致敏RBL-2H3细胞的实验研究[J].中国实验方剂学杂志,2013,19(03):245-248. DOI： 10.13422/j.cnki.syfjx.2013.03.079.

YU Hai-bin1, LIU Qing2, CUI Lin1. Study of Mailuoning Cause an Allergic the RBL-2H3 Cells[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(3): 245-248. DOI： 10.13422/j.cnki.syfjx.2013.03.079.

摘要

目的:通过研究临床含有绿原酸的中药注射剂脉络宁针剂对RBL-2H3细胞（大鼠嗜碱性白血病细胞系）脱颗粒、释放介质的影响

探讨绿原酸与中药注射剂发生严重过敏反应的关系

为临床安全用药提供依据。方法:以含有绿原酸的中药针剂脉络宁为研究对象

以不含绿原酸的葛根素针剂为阴性对照

以绿原酸对照品为阳性对照

通过对体外培养RBL-2H3细胞模型的作用

观察该细胞模型发生脱颗粒反应和细胞形态变化

检测β-己糖胺酶释放量。结果:脉络宁、绿原酸组可显著引起RBL-2H3细胞释放β-己糖胺酶（P<0.05）;葛根素注射液组能不同程度促进β-己糖胺酶的释放。结论:脉络宁组可显著引起RBL-2H3细胞释放β-己糖胺酶

提示绿原酸与中药注射剂脉络宁发生严重过敏反应有密切关系。

Abstract

Objective:Relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection was studied by observing influnce of Mailuoning injection on release media of RBL-2H3 cell（rat basophilic leukemia cell line）.Method: Mailuoning containing chlorogenic acid was as a suject

and puerarin injection not containing as a negative control

while chlorogenic acid standard as a positive control

the influntion of RBL-2H3 cells model in vitro

the degranulation response and changes in cell morphology was observed

β-hexosaminidase release was detected.Result: The Mailuoning and chlorogenic acid can be a significant factor resulting in release of β-hexosaminidase from RBL-2H3 cells（n=5

P<0.05）

puerarin injection group could promote the release of β-hexosaminidase.Conclusion: The Mailuoning group abversely caused the RBL-2H3 cells releasing β-hexosaminidase

and there is some relativity between the severe allergic reaction of the chlorogenic acid and traditional chinese medicine injection.

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