Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD

YANG Yue-e; ZHU En-huan; HU Wen; YUAN Yun; MA Xing-fen; LIU De-ju

doi:10.13422/j.cnki.syfjx.2013.05.002

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Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD

更新时间：2024-09-23

- Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 120-122(2013)
- 作者机构：
  
  大理学院药学院
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.002
  CLC： O657.72;TS251.7
- Published：2013
- 稿件说明：
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[1]杨月娥,朱恩焕,胡文,袁云,马兴芬,刘德举.HPLC-DAD同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量[J].中国实验方剂学杂志,2013,19(05):120-122.

YANG Yue-e, ZHU En-huan, HU Wen, et al. Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 120-122.
[1]杨月娥,朱恩焕,胡文,袁云,马兴芬,刘德举.HPLC-DAD同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量[J].中国实验方剂学杂志,2013,19(05):120-122. DOI： 10.13422/j.cnki.syfjx.2013.05.002.

YANG Yue-e, ZHU En-huan, HU Wen, et al. Simultaneous Determination of Rutin,Quercetin and Quercitroside in Incarvillea mairei by HPLC-DAD[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 120-122. DOI： 10.13422/j.cnki.syfjx.2013.05.002.

摘要

目的:建立同时测定鸡肉参中芦丁、槲皮苷、槲皮素含量的HPLC-DAD方法。方法:采用Diamonsil-C18色谱柱（150 mm×4.6 mm

5.0μm）

柱温30℃

以甲醇（A）-0.5%磷酸水溶液（B）为流动相

进行梯度洗脱（0～5 min

48%～52%A;6～10 min

70%～30%A）

检测波长254 nm

流速1.0 mL.min-1。结果:3种黄酮类成分在10 min内达到良好分离

芦丁、槲皮苷、槲皮素的线性范围分别为0.3～1.0 mg（r=0.999 8）

0.030～0.10 mg（r=0.999 8）

0.010～0.033 mg（r=0.999 5）;平均加样回收率分别为100.95%（RSD 0.98%）

99.51%（RSD 1.54%）

102.84%（RSD 3.53%）。结论:该方法简便

快速

精确

可同时测定鸡肉参中芦丁、槲皮苷、槲皮素的含量

具有较好的重复性和稳定性

可作为鸡肉参药材的质量控制方法。

Abstract

Objective:To establish an HPLC-DAD method for simultaneous determination of rutin

quercetin and quercitroside in Incarvillea mairei.Method: A Dikma Diamonsil C18column（4.6 mm×150 mm

5 μm） was used.The column temperature was set at 30 ℃;the investigated compounds were separated with gradient mobile phase consisting of methanol（A）-0.5% phosphoric acid（B）;the flow rate was 1.0 mL·min-1 and the detection wavelength was kept at 254 nm.Result: The good separation of three flavonoids was achieved within 10 min.The linearity was achieved in the range of 0.30-1.0 mg（r=0.999 8） for rutin

0.030-0.10 mg（r=0.999 8） for quercetin and 0.010-0.033 mg（r=0.999 5） for quercitrosid.The average recoveries were 100.95%（RSD 0.98%）

99.51%（RSD 1.54%） and 102.84%（RSD 3.53%） respectively.Conclusion: The method is simple

rapid and accurate

which can be able to detect rutin

quercetin and quercitroside simultaneously

and can be used for quality control of I.mairei.

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Related Author

YANG Yue-e

ZHU En-huan

HU Wen

YUAN Yun

MA Xing-fen

LIU De-ju

YUE Xiao-hua

SHI Jing-chao

Related Institution

Beijing Research Institute of Chinese Medicine， Beijing University of Chinese Medicine

College of Life Sciences， Beijing University of Chinese Medicine

College of Chinese Materia Medica， Beijing University of Chinese Medicine

College of Pharmacy, Shaanxi University of Chinese Medicine

Guizhou Provincial Engineering Center of Efficient Utilization of Natural Medicinal Resources, High Educational Key Laboratory of Natural Medicinal Pharmacology and Druggability of Guizhou Province, Joint Key Laboratory of Guizhou Medical University-Guiyang City, Key Laboratory of Optimal Utilization of Natural Medicinal Resources, School of Pharmaceutical Sciences, Guizhou Medical University

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