LIAO Chang-neng1, PAN Yong2. Determination of Umbelliferone in Dichondra repens of Traditional Zhuang Medicine[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 149-151.
LIAO Chang-neng1, PAN Yong2. Determination of Umbelliferone in Dichondra repens of Traditional Zhuang Medicine[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 149-151. DOI: 10.13422/j.cnki.syfjx.2013.05.013.
Determination of Umbelliferone in Dichondra repens of Traditional Zhuang Medicine
摘要
目的:建立马蹄金中伞形花内酯的含量测定方法。方法:采用VP-ODS C18(4.6 mm×150 mm
Objective:To establish the determination method of umbelliferone content in Dichondra repens.Method: VP-ODS Cl8(4.6 mm×150 mm
5 μm) column was used.The mobile phase was methanol-tetrahydrofuran-water solution(15∶85);the flow rate was 1.0 mL·min-1;the detection wavelength was at 325 nm.Result: The linear relation of hyperin was good in the range from 0.050 7 to 0.507 0 μg.The average recovery was 98.85%
RSD was 1.00%.Conclusion: The method established is accurate
sensitive and good in reproducibility.It can be applied to the quality control of D.repens.