Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes

WANG An-yuan1; LING Jia-jun1; XU Yue-yuan1; XIE Bo2; HAO Jian-dong3

doi:10.13422/j.cnki.syfjx.2013.05.039

您当前的位置：

首页 >

文章列表页 >

Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes

更新时间：2024-09-23

- Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 36-39(2013)
- 作者机构：
  
  1. 广州中医药大学中药学院
  2. 广州军区广州总医院
  3. 广州意斯生物工程科技有限公司
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.039
  CLC： R283
- Published：2013
- 稿件说明：
移动端阅览
[1]王安元,凌家俊,徐月圆,谢波,郝建冬.二氢卟吩e6脂质体的制备及影响因素考察[J].中国实验方剂学杂志,2013,19(05):36-39.

WANG An-yuan1, LING Jia-jun1, XU Yue-yuan1, et al. Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 36-39.
[1]王安元,凌家俊,徐月圆,谢波,郝建冬.二氢卟吩e6脂质体的制备及影响因素考察[J].中国实验方剂学杂志,2013,19(05):36-39. DOI： 10.13422/j.cnki.syfjx.2013.05.039.

WANG An-yuan1, LING Jia-jun1, XU Yue-yuan1, et al. Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 36-39. DOI： 10.13422/j.cnki.syfjx.2013.05.039.

摘要

目的:建立二氢卟吩e6脂质体包封率的测定方法

考察各因素对包封率的影响。方法:分别采用Sephadex G50

Sephadex G75凝胶柱分离脂质体和游离药物

优选测定二氢卟吩e6脂质体包封率的方法;以包封率为指标

采用单因素试验考察磷脂质量浓度、胆固醇用量、水化介质、温度及时间对二氢卟吩e6脂质体制备的影响。结果:选择Sephadex G50柱色谱法测定二氢卟吩e6的包封率

其对游离药物回收率达100.6%

采用该法测定包封率的重复性RSD<0.02;磷脂与胆固醇的质量比、药脂比、水化介质对包封率的影响较大。结论:SephadexG50凝胶柱色谱法能方便、快速地测定二氢卟吩e6脂质体的包封率

建立的方法稳定可行。

Abstract

Objective:To establish a method for determining entrapment efficiency（EE） of chlorin e6 liposomes

and to investigate effect of different factors on it.Method:Sephadex G50 and Sephadex G75 column filtration methods were used to separate free drug and liposomes

in order to optimize a method to determine EE of chlorin e6 liposomes;With EE as index

influence of phospholipid concentration

cholesterol amount

hydration medium

temperature and time on preparation of chlorin e6 liposomes were investigated by single factor test.Result:EE of chlorin e6 liposomes was determined by Sephadex G50 column chromatography

its recovery for free drug was up to 100.6%

RSD of repeatability test was less than 0.02;Ratio of phospholipid-cholesterol

drug-lipid ratio and hydration medium had great effect on EE.Conclusion:Sephadex G50 column filtration method was a good way to determine EE of chlorin e6 liposomes easily and quickly

established method was stable and feasible.

关键词

Keywords

references

Views

291

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Preparation and Factors Investigation Affecting Entrapment Efficiency of Chlorin e6 Liposomes

Determination of Content and Entrapment Efficiency of Vinorelbine Encapsulated in Liposomes

Extraction process of Volatile Oils and -cyclodextrin Inclusion of Changweisan Tablets

Content Determination and Entrapment Efficiency Investigation of Gentiopicroside Lipsome

Related Author

WANG An-yuan

LING Jia-jun

XU Yue-yuan

XIE Bo

HAO Jian-dong

LI Xin

CHENG Lan

LI Xue-tao

Related Institution

Guangdong Pharmaceutical University

The Medicine Institute of Liaoning University of Traditional Chinese Medicine

AI问答

Postal code：100700
Tel：010-84076882 Email：syfjx_2010@188.com
Technical support is provided by Beijing Founder electronics co., LTD 京ICP备11006657号-10 京公网安备11010802024621
It is recommended to read the content of this site in Chrome&IE9+. Please switch to extreme mode in browser 360.
Cookies We use cookies to help provide and enhance our service and tailor content. By continuing, you agree to the use of cookies.

⁰