Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong

YIN Ling; CHEN Hong-ping; PENG Yue; LIU Rong; FAN Dan-qing; LIU You-ping

doi:10.13422/j.cnki.syfjx.2013.05.041

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Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong

更新时间：2024-09-23

- Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong
- Chinese Journal of Experimental Traditional Medical Formulae Vol. 19, Issue 5, Pages: 42-46(2013)
- 作者机构：
  
  成都中医药大学药学院,中药资源系统研究与开发利用省部共建国家重点实验室培育基地
- 作者简介：
- 基金信息：
- DOI：10.13422/j.cnki.syfjx.2013.05.041
  CLC： R284
- Published：2013
- 稿件说明：
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[1]银玲,陈鸿平,彭月,刘荣,樊丹青,刘友平.川芎中总荧光物质的含量测定及提取工艺优化[J].中国实验方剂学杂志,2013,19(05):42-46.

YIN Ling, CHEN Hong-ping, PENG Yue, et al. Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 42-46.
[1]银玲,陈鸿平,彭月,刘荣,樊丹青,刘友平.川芎中总荧光物质的含量测定及提取工艺优化[J].中国实验方剂学杂志,2013,19(05):42-46. DOI： 10.13422/j.cnki.syfjx.2013.05.041.

YIN Ling, CHEN Hong-ping, PENG Yue, et al. Content Determination and Extraction Technology Optimization of Total Fluorescent Substances from Ligusticum chuanxiong[J]. Chinese journal of experimental traditional medical formulae, 2013, 19(5): 42-46. DOI： 10.13422/j.cnki.syfjx.2013.05.041.

摘要

目的:建立川芎中总荧光物质的含量测定方法

优化总荧光物质的超声提取工艺。方法:采用荧光分光光度法测定川芎中总荧光物质的含量。通过单因素试验考察料液比、超声时间、提取溶剂种类与浓度对总荧光物质提取工艺的影响

采用星点设计对总荧光物质超声提取工艺的参数进行优选

通过对自变量各水平的多元线性回归及二项式拟合

选用效应面法选取最佳工艺

并进行预测分析。结果:确定的最佳工艺条件为乙醇体积分数63%

液固比350∶1

超声53 min。川芎中总荧光物质提取率的预测值达3.444%

预测值与验证值偏差2.70%。二项式拟合R2=0.953 2。结论:优选的提取工艺具有简便、精密度高、可预测性好的优点;建立的含量测定方法稳定可行

为川芎药材的质量控制提供理论依据。

Abstract

Objective:To establish content determination method of total fluorescent substances from Ligusticum chuanxiong

and optimize ultrasonic extraction technology of total fluorescent substances.Method:Fluorescence spectrophotometric method was adopted for determining the content of total fluorescent substances from L.chuanxiong.Influence of solid-liquid ratio

extraction time

kind and concentration of extraction solvent on extraction technology of total fluorescent substances was investigated by single factor test

ultrasound extraction technology parameters of total fluorescent substances were optimized by composite design

multiple linear regression and binomial fitting were used to evaluate relationship between independent and dependent variables

response surface methodology was used to optimize extraction process

prediction analysis was carried out by comparing the observed value and the predicted value.Result:Optimal technology conditions of ultrasonic extraction process were as followings:ultrasound extracted 53 min with 350 times the amount of 63% ethanol.The predictive extraction rate of total fluorescent substances from L.chuanxiong was 3.444%

bias between the observed value and the predicted value was 2.70%.Regression coefficients of binomial fitting complex model was as high as 0.953 2.Conclusion:This optimized ultrasonic extraction technology was simple with high precision and good predictability.Established content determination method was stable and feasible

it could provide a theoretical basis for quality control of L.chuanxiong.

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YIN Ling

CHEN Hong-ping

PENG Yue

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FAN Dan-qing

LIU You-ping

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Related Institution

Heilongjiang University of Chinese Medicine

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